Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups
The study investigated the effect of buprenorphine (BUP) on oxidative indices and gene expression of apoptotic molecules in the hippocampus of neonates during the fetal stage. BUP (1 or 0.5 mg/kg) was subcutaneously administrated to pregnant rat dams. After parturition, the pups were maintained to t...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022000300 |
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| author | Saeed Samarghandian Fahimeh Ghasemi Hamed Aramjoo Fariborz Samini Michael Aschner Babak Roshanravan Tahereh Farkhondeh |
| author_facet | Saeed Samarghandian Fahimeh Ghasemi Hamed Aramjoo Fariborz Samini Michael Aschner Babak Roshanravan Tahereh Farkhondeh |
| author_sort | Saeed Samarghandian |
| collection | DOAJ |
| description | The study investigated the effect of buprenorphine (BUP) on oxidative indices and gene expression of apoptotic molecules in the hippocampus of neonates during the fetal stage. BUP (1 or 0.5 mg/kg) was subcutaneously administrated to pregnant rat dams. After parturition, the pups were maintained to the end of breastfeeding period, then hippocampi were assessed for oxidative stress indices [glutathione (GSH), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), total antioxidant capacity (TAC)] and mRNA expression of apoptotic markers (Bax, Bcl2 and caspase 3). Our data indicated that BUP (0.5 mg/kg) administration during gestation significantly increased GSH and TAC concentrations in the hippocampus of pups versus control group (p < 0.05). BUP (0.5 and 1 mg/kg) administration significantly elevated the expression levels of Bcl2 in the hippocampus of neonates compared with controls. BUP injection (0.5 and 1 mg/kg) to pregnant rats markedly reduced the expression levels of caspase 3 in the hippocampus of neonates in BUP 0.5 group (p < 0.01) and BUP 1 group (p < 0.05) versus the controls. Our study indicated that BUP may potentiate antioxidant system and inhibit apoptosis and oxidative stress in the hippocampus of neonates received this drug during the fetal stage. |
| first_indexed | 2024-04-13T05:10:04Z |
| format | Article |
| id | doaj.art-588583dc799c415a843251892ce8b14e |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-13T05:10:04Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-588583dc799c415a843251892ce8b14e2022-12-22T03:01:04ZengElsevierToxicology Reports2214-75002022-01-019311315Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pupsSaeed Samarghandian0Fahimeh Ghasemi1Hamed Aramjoo2Fariborz Samini3Michael Aschner4Babak Roshanravan5Tahereh Farkhondeh6Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, IranCellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran; Department of Biotechnology, School of Medicine, Birjand University of Medical Sciences, Birjand, IranMedical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences (BUMS), Birjand, IranNeuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Neurosurgery, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Molecular Pharmacology, Albert Einstein College of Medicine, Forchheimer 209, 1300 Morris Park Avenue, Bronx, NY 10461, USAStudent Research Committee, Birjand University of Medical Sciences, Birjand, IranMedical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences (BUMS), Birjand, Iran; Faculty of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran; Correspondence to: Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences, Birjand, Iran. Faculty of Pharmacy, Birjand University of Medical Sciences, Birjand, Iran.The study investigated the effect of buprenorphine (BUP) on oxidative indices and gene expression of apoptotic molecules in the hippocampus of neonates during the fetal stage. BUP (1 or 0.5 mg/kg) was subcutaneously administrated to pregnant rat dams. After parturition, the pups were maintained to the end of breastfeeding period, then hippocampi were assessed for oxidative stress indices [glutathione (GSH), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), total antioxidant capacity (TAC)] and mRNA expression of apoptotic markers (Bax, Bcl2 and caspase 3). Our data indicated that BUP (0.5 mg/kg) administration during gestation significantly increased GSH and TAC concentrations in the hippocampus of pups versus control group (p < 0.05). BUP (0.5 and 1 mg/kg) administration significantly elevated the expression levels of Bcl2 in the hippocampus of neonates compared with controls. BUP injection (0.5 and 1 mg/kg) to pregnant rats markedly reduced the expression levels of caspase 3 in the hippocampus of neonates in BUP 0.5 group (p < 0.01) and BUP 1 group (p < 0.05) versus the controls. Our study indicated that BUP may potentiate antioxidant system and inhibit apoptosis and oxidative stress in the hippocampus of neonates received this drug during the fetal stage.http://www.sciencedirect.com/science/article/pii/S2214750022000300BuprenorphineApoptosisOxidative stressBrainRatPup |
| spellingShingle | Saeed Samarghandian Fahimeh Ghasemi Hamed Aramjoo Fariborz Samini Michael Aschner Babak Roshanravan Tahereh Farkhondeh Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups Toxicology Reports Buprenorphine Apoptosis Oxidative stress Brain Rat Pup |
| title | Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups |
| title_full | Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups |
| title_fullStr | Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups |
| title_full_unstemmed | Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups |
| title_short | Effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups |
| title_sort | effects of exposure in utero to buprenorphine on oxidative stress and apoptosis in the hippocampus of rat pups |
| topic | Buprenorphine Apoptosis Oxidative stress Brain Rat Pup |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022000300 |
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