Isolation and transfection of myenteric neurons from mice for patch-clamp applications

Isolation and transfection of myenteric neurons from mice for patch-clamp applications

The enteric nervous system (ENS) is a complex neuronal network organized in ganglionated plexuses that extend along the entire length of the gastrointestinal tract. Largely independent of the central nervous system, the ENS coordinates motility and peristalsis of the digestive tract, regulates secre...

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Bibliographic Details
Main Authors: Samuel Kuehs, Laura Teege, Ann-Katrin Hellberg, Christina Stanke, Natja Haag, Ingo Kurth, Robert Blum, Carla Nau, Enrico Leipold
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Molecular Neuroscience
Subjects:
single cell isolation
sodium channels
NaV1.9
patch-clamp
electrophysiology
myenteric neurons
Online Access:https://www.frontiersin.org/articles/10.3389/fnmol.2022.1076187/full

Internet

https://www.frontiersin.org/articles/10.3389/fnmol.2022.1076187/full

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