Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice
Gui-ling-gao (GLG), also known as turtle jelly, is a popular medicinal Chinese health/functional food prepared from several traditional Chinese medicinal herbs. The present study aimed at examining and explaining the anti-inflammatory properties of GLG by using lipopolysaccharide (LPS)-induced infla...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464613000066 |
| _version_ | 1818958141443801088 |
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| author | Huan Zhang Min-Yi Wu De-Jian Guo Chun-Wai Wan Ching-Ching Lau Chi-On Chan Daniel Kam-Wah Mok Shun-Wan Chan |
| author_facet | Huan Zhang Min-Yi Wu De-Jian Guo Chun-Wai Wan Ching-Ching Lau Chi-On Chan Daniel Kam-Wah Mok Shun-Wan Chan |
| author_sort | Huan Zhang |
| collection | DOAJ |
| description | Gui-ling-gao (GLG), also known as turtle jelly, is a popular medicinal Chinese health/functional food prepared from several traditional Chinese medicinal herbs. The present study aimed at examining and explaining the anti-inflammatory properties of GLG by using lipopolysaccharide (LPS)-induced inflammation in splenocytes isolated from BALB/c mice. Additionally, the effects of GLG on mRNA and protein expressions of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in LPS-stimulated splenocyte proliferation were evaluated by real-time RT-PCR and Western blot assays. We demonstrated that GLG significantly inhibited LPS-induced splenocyte proliferation in a concentration-dependent manner. LPS-mediated up-regulations in the gene and protein expressions of iNOS, interleukin-1β (IL-1β) and tumour necrosis factor-alpha (TNF-α) were suppressed by GLG. These findings suggest for the first time that GLG exerts anti-inflammatory effects by inhibiting the expression of iNOS and pro-inflammatory cytokines such as IL-1β and TNF-α. The current study provides strong scientific evidence for the health-beneficial claim that consumption of GLG could help the body to modulate the immune system. |
| first_indexed | 2024-12-20T11:21:02Z |
| format | Article |
| id | doaj.art-58bc47339100481399a8b65c2fbd47c7 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T11:21:02Z |
| publishDate | 2013-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-58bc47339100481399a8b65c2fbd47c72022-12-21T19:42:31ZengElsevierJournal of Functional Foods1756-46462013-04-0152625632Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c miceHuan Zhang0Min-Yi Wu1De-Jian Guo2Chun-Wai Wan3Ching-Ching Lau4Chi-On Chan5Daniel Kam-Wah Mok6Shun-Wan Chan7Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR ChinaState Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR ChinaState Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR ChinaDepartment of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR ChinaDepartment of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR ChinaState Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR ChinaState Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR ChinaFood Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China; Corresponding author at: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, PR China. Tel.: +852 34008718; fax: +852 23649932.Gui-ling-gao (GLG), also known as turtle jelly, is a popular medicinal Chinese health/functional food prepared from several traditional Chinese medicinal herbs. The present study aimed at examining and explaining the anti-inflammatory properties of GLG by using lipopolysaccharide (LPS)-induced inflammation in splenocytes isolated from BALB/c mice. Additionally, the effects of GLG on mRNA and protein expressions of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in LPS-stimulated splenocyte proliferation were evaluated by real-time RT-PCR and Western blot assays. We demonstrated that GLG significantly inhibited LPS-induced splenocyte proliferation in a concentration-dependent manner. LPS-mediated up-regulations in the gene and protein expressions of iNOS, interleukin-1β (IL-1β) and tumour necrosis factor-alpha (TNF-α) were suppressed by GLG. These findings suggest for the first time that GLG exerts anti-inflammatory effects by inhibiting the expression of iNOS and pro-inflammatory cytokines such as IL-1β and TNF-α. The current study provides strong scientific evidence for the health-beneficial claim that consumption of GLG could help the body to modulate the immune system.http://www.sciencedirect.com/science/article/pii/S1756464613000066Turtle jellyLipopolysaccharideAnti-inflammatoryPro-inflammatory cytokinesSplenocytes |
| spellingShingle | Huan Zhang Min-Yi Wu De-Jian Guo Chun-Wai Wan Ching-Ching Lau Chi-On Chan Daniel Kam-Wah Mok Shun-Wan Chan Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice Journal of Functional Foods Turtle jelly Lipopolysaccharide Anti-inflammatory Pro-inflammatory cytokines Splenocytes |
| title | Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice |
| title_full | Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice |
| title_fullStr | Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice |
| title_full_unstemmed | Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice |
| title_short | Gui-ling-gao (turtle jelly), a traditional Chinese functional food, exerts anti-inflammatory effects by inhibiting iNOS and pro-inflammatory cytokine expressions in splenocytes isolated from BALB/c mice |
| title_sort | gui ling gao turtle jelly a traditional chinese functional food exerts anti inflammatory effects by inhibiting inos and pro inflammatory cytokine expressions in splenocytes isolated from balb c mice |
| topic | Turtle jelly Lipopolysaccharide Anti-inflammatory Pro-inflammatory cytokines Splenocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1756464613000066 |
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