The ATP/Mg<sup>2+</sup> Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome

The ATP/Mg<sup>2+</sup> Balance Affects the Degradation of Short Fluorogenic Substrates by the 20S Proteasome

Proteasomes hydrolyze most cellular proteins. The standard reaction to determine proteasome activity in cellular lysate or elsewhere contains AMC-conjugated peptide substrate, ATP, Mg<sup>2+</sup>, and DTT. ATP and Mg<sup>2+</sup> are included to maintain 26S proteasome funct...

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Bibliographic Details
Main Authors: Alexey Morozov, Tatyana Astakhova, Pavel Erokhov, Vadim Karpov
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Methods and Protocols
Subjects:
proteasome
immunoproteasome
proteasome activity
fluorogenic substrates
Online Access:https://www.mdpi.com/2409-9279/5/1/15

Internet

https://www.mdpi.com/2409-9279/5/1/15

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