Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs

Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs

Abstract Synthetic sRNAs allow knockdown of target genes at translational level, but have been restricted to a limited number of bacteria. Here, we report the development of a broad-host-range synthetic sRNA (BHR-sRNA) platform employing the RoxS scaffold and the Hfq chaperone from Bacillus subtilis...

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Bibliographic Details
Main Authors: Jae Sung Cho, Dongsoo Yang, Cindy Pricilia Surya Prabowo, Mohammad Rifqi Ghiffary, Taehee Han, Kyeong Rok Choi, Cheon Woo Moon, Hengrui Zhou, Jae Yong Ryu, Hyun Uk Kim, Sang Yup Lee
Format: Article
Language:English
Published: Nature Portfolio 2023-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-38119-y

Internet

https://doi.org/10.1038/s41467-023-38119-y

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