Efficacy of genetic transformation of E.coli field and reference (ATCC 8739) strains at different concentrations of CaCl2 for creation of Gene libraries

The importance of genetic transformation in various fields of biological research, and especially in the process of gene libraries formation, is very important. It has been proven by numerous studies that during the phase of obtaining competent cells, Ca2+ ions neutralize the electrostatic repulsion...

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Bibliographic Details
Main Authors: Badalyan Manvel, Aloyan Tatevik, Amirkhanyan Tatev, Dilanyan Varya, Melikyan Andreas
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2023/16/bioconf_cibta2023_01082.pdf