Insights regarding sirtuin-dependent gene regulation during white koji production
White koji, a solid-state culture of Aspergillus luchuensis mut. kawachii using grains such as rice and barley, is used as a source of amylolytic enzymes and citric acid for the production of shochu, a traditional Japanese distilled spirit. We previously characterized changes in gene expression that...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Communicative & Integrative Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19420889.2022.2051844 |
| _version_ | 1818156334853390336 |
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| author | Taiki Futagami Masatoshi Goto |
| author_facet | Taiki Futagami Masatoshi Goto |
| author_sort | Taiki Futagami |
| collection | DOAJ |
| description | White koji, a solid-state culture of Aspergillus luchuensis mut. kawachii using grains such as rice and barley, is used as a source of amylolytic enzymes and citric acid for the production of shochu, a traditional Japanese distilled spirit. We previously characterized changes in gene expression that affect the properties of white koji during the shochu production process; however, the underlying regulatory mechanisms were not determined. We then characterized the NAD+-dependent histone deacetylase sirtuin, an epigenetic regulator of various biological phenomena, in A. l. mut. kawachii and found that sirtuin SirD is involved in expression of α-amylase activity and citric acid accumulation. In this addendum study, we measured the NAD+/NADH redox state and found that the NAD+ level and NAD+/NADH ratio decrease during koji production, indicating that sirtuin activity declines in the late stages of koji culture. By comparing these results with transcriptomic data obtained in our previous studies, we estimate that approximately 35% of the gene expression changes during white koji production are SirD dependent. This study provides clues to the mechanism of gene expression regulation in A. l. mut. kawachii during the production of white koji. |
| first_indexed | 2024-12-11T14:56:39Z |
| format | Article |
| id | doaj.art-58eb7e8422a84870ae775a508066fe63 |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-11T14:56:39Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-58eb7e8422a84870ae775a508066fe632022-12-22T01:01:18ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892022-12-01151929510.1080/19420889.2022.2051844Insights regarding sirtuin-dependent gene regulation during white koji productionTaiki Futagami0Masatoshi Goto1Education and Research Center for Fermentation Studies, Faculty of Agriculture, Kagoshima University, Kagoshima, JapanUnited Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, JapanWhite koji, a solid-state culture of Aspergillus luchuensis mut. kawachii using grains such as rice and barley, is used as a source of amylolytic enzymes and citric acid for the production of shochu, a traditional Japanese distilled spirit. We previously characterized changes in gene expression that affect the properties of white koji during the shochu production process; however, the underlying regulatory mechanisms were not determined. We then characterized the NAD+-dependent histone deacetylase sirtuin, an epigenetic regulator of various biological phenomena, in A. l. mut. kawachii and found that sirtuin SirD is involved in expression of α-amylase activity and citric acid accumulation. In this addendum study, we measured the NAD+/NADH redox state and found that the NAD+ level and NAD+/NADH ratio decrease during koji production, indicating that sirtuin activity declines in the late stages of koji culture. By comparing these results with transcriptomic data obtained in our previous studies, we estimate that approximately 35% of the gene expression changes during white koji production are SirD dependent. This study provides clues to the mechanism of gene expression regulation in A. l. mut. kawachii during the production of white koji.https://www.tandfonline.com/doi/10.1080/19420889.2022.2051844Aspergillus luchuensis mut. kawachiiwhite kojigene regulationNAD+-dependent histone deacetylasesirtuin |
| spellingShingle | Taiki Futagami Masatoshi Goto Insights regarding sirtuin-dependent gene regulation during white koji production Communicative & Integrative Biology Aspergillus luchuensis mut. kawachii white koji gene regulation NAD+-dependent histone deacetylase sirtuin |
| title | Insights regarding sirtuin-dependent gene regulation during white koji production |
| title_full | Insights regarding sirtuin-dependent gene regulation during white koji production |
| title_fullStr | Insights regarding sirtuin-dependent gene regulation during white koji production |
| title_full_unstemmed | Insights regarding sirtuin-dependent gene regulation during white koji production |
| title_short | Insights regarding sirtuin-dependent gene regulation during white koji production |
| title_sort | insights regarding sirtuin dependent gene regulation during white koji production |
| topic | Aspergillus luchuensis mut. kawachii white koji gene regulation NAD+-dependent histone deacetylase sirtuin |
| url | https://www.tandfonline.com/doi/10.1080/19420889.2022.2051844 |
| work_keys_str_mv | AT taikifutagami insightsregardingsirtuindependentgeneregulationduringwhitekojiproduction AT masatoshigoto insightsregardingsirtuindependentgeneregulationduringwhitekojiproduction |