An Advanced Multiplex Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Reliable Detection of Porcine Epidemic Diarrhea Virus and Porcine Internal Positive Control

An Advanced Multiplex Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Reliable Detection of Porcine Epidemic Diarrhea Virus and Porcine Internal Positive Control

For rapid and reliable detection of porcine epidemic diarrhea virus (PEDV) from pig clinical samples, a multiplex, real-time, reverse transcription loop-mediated isothermal amplification (mqRT-LAMP) was developed using two sets of primers and assimilating probes specific to the PEDV N gene and the &...

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Bibliographic Details
Main Authors: Hye-Ryung Kim, Jong-Min Kim, Ji-Su Baek, Jonghyun Park, Won-Il Kim, Bok Kyung Ku, Hye-Young Jeoung, Kyoung-Ki Lee, Choi-Kyu Park
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Viruses
Subjects:
PEDV
multiplex real-time RT-LAMP
assimilating probe
N gene
<i>Sus scrofa β-actin</i> gene
internal positive control
Online Access:https://www.mdpi.com/1999-4915/15/11/2204

Internet

https://www.mdpi.com/1999-4915/15/11/2204

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