Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese
<i>Listeria monocytogenes</i> is one of the most important emerging foodborne pathogens. The objectives of this work were to investigate the incidence of <i>Listeria</i> spp. and <i>L. monocytogenes</i> in soft cheese and ice cream in Assiut city, Egypt, and to ex...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/21/3443 |
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| author | Rania M. Ewida Walaa S. Hasan Mohamed Salem Elfaruk Raed Reshaid Alayouni Ahmed R. A. Hammam Dalia G. Kamel |
| author_facet | Rania M. Ewida Walaa S. Hasan Mohamed Salem Elfaruk Raed Reshaid Alayouni Ahmed R. A. Hammam Dalia G. Kamel |
| author_sort | Rania M. Ewida |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> is one of the most important emerging foodborne pathogens. The objectives of this work were to investigate the incidence of <i>Listeria</i> spp. and <i>L. monocytogenes</i> in soft cheese and ice cream in Assiut city, Egypt, and to examine the effect of some probiotic <i>Bifidobacterium</i> spp. (<i>Bifidobacterium breve</i>, <i>Bifidobacterium animalis</i>, or a mixture of the two) on the viability of <i>L. monocytogenes</i> in soft cheese. The existence of <i>Listeria</i> spp. and <i>L. monocytogenes</i> was examined in 30 samples of soft cheese and 30 samples of ice cream. Bacteriological analyses and molecular identification (using 16S rRNA gene and <i>hlyA</i> gene for <i>Listeria</i> spp. and <i>L. monocytogenes</i>, respectively) were performed on those samples. Additionally, <i>Bifidobacterium</i> spp. were incorporated in the making of soft cheese to study their inhibitory impacts on <i>L. monocytogenes</i>. Out of 60 samples of soft cheese and ice cream, 25 samples showed <i>Listeria</i> spp., while <i>L. monocytogenes</i> was found in only 2 soft cheese samples. Approximately 37% of soft cheese samples (11 out of 30) had <i>Listeria</i> spp. with about 18.0% (2 out of 11) exhibiting <i>L. monocytogenes</i>. In ice cream samples, <i>Listeria</i> spp. was presented by 47% (14 out of 30), while <i>L. monocytogenes</i> was not exhibited. Moreover, the addition of <i>B. animalis</i> to soft cheese in a concentration of 5% or combined with <i>B. breve</i> with a concentration of 2.5% for each resulted in decreasing <i>L. monocytogenes</i> efficiently during the ripening of soft cheese for 28 d. <i>Listeria</i> spp. is widely found in milk products. Probiotic bacteria, such as <i>Bifidobacterium</i> spp., can be utilized as a natural antimicrobial to preserve food and dairy products. |
| first_indexed | 2024-03-09T19:04:26Z |
| format | Article |
| id | doaj.art-58f3693bdc454f70ac905e4cc60b1bae |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:04:26Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-58f3693bdc454f70ac905e4cc60b1bae2023-11-24T04:40:54ZengMDPI AGFoods2304-81582022-10-011121344310.3390/foods11213443Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft CheeseRania M. Ewida0Walaa S. Hasan1Mohamed Salem Elfaruk2Raed Reshaid Alayouni3Ahmed R. A. Hammam4Dalia G. Kamel5Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, New Valley University, Kharga 72511, EgyptCollege of Milk Hygiene, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, EgyptDairy and Food Science Department, South Dakota State University, Brookings, SD 57007, USADairy and Food Science Department, South Dakota State University, Brookings, SD 57007, USADairy and Food Science Department, South Dakota State University, Brookings, SD 57007, USADairy Science Department, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt<i>Listeria monocytogenes</i> is one of the most important emerging foodborne pathogens. The objectives of this work were to investigate the incidence of <i>Listeria</i> spp. and <i>L. monocytogenes</i> in soft cheese and ice cream in Assiut city, Egypt, and to examine the effect of some probiotic <i>Bifidobacterium</i> spp. (<i>Bifidobacterium breve</i>, <i>Bifidobacterium animalis</i>, or a mixture of the two) on the viability of <i>L. monocytogenes</i> in soft cheese. The existence of <i>Listeria</i> spp. and <i>L. monocytogenes</i> was examined in 30 samples of soft cheese and 30 samples of ice cream. Bacteriological analyses and molecular identification (using 16S rRNA gene and <i>hlyA</i> gene for <i>Listeria</i> spp. and <i>L. monocytogenes</i>, respectively) were performed on those samples. Additionally, <i>Bifidobacterium</i> spp. were incorporated in the making of soft cheese to study their inhibitory impacts on <i>L. monocytogenes</i>. Out of 60 samples of soft cheese and ice cream, 25 samples showed <i>Listeria</i> spp., while <i>L. monocytogenes</i> was found in only 2 soft cheese samples. Approximately 37% of soft cheese samples (11 out of 30) had <i>Listeria</i> spp. with about 18.0% (2 out of 11) exhibiting <i>L. monocytogenes</i>. In ice cream samples, <i>Listeria</i> spp. was presented by 47% (14 out of 30), while <i>L. monocytogenes</i> was not exhibited. Moreover, the addition of <i>B. animalis</i> to soft cheese in a concentration of 5% or combined with <i>B. breve</i> with a concentration of 2.5% for each resulted in decreasing <i>L. monocytogenes</i> efficiently during the ripening of soft cheese for 28 d. <i>Listeria</i> spp. is widely found in milk products. Probiotic bacteria, such as <i>Bifidobacterium</i> spp., can be utilized as a natural antimicrobial to preserve food and dairy products.https://www.mdpi.com/2304-8158/11/21/3443listeriosisprobiotic<i>Bifidobacterium</i>dairy productspreservativesinhibition |
| spellingShingle | Rania M. Ewida Walaa S. Hasan Mohamed Salem Elfaruk Raed Reshaid Alayouni Ahmed R. A. Hammam Dalia G. Kamel Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese Foods listeriosis probiotic <i>Bifidobacterium</i> dairy products preservatives inhibition |
| title | Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese |
| title_full | Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese |
| title_fullStr | Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese |
| title_full_unstemmed | Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese |
| title_short | Occurrence of <i>Listeria</i> spp. in Soft Cheese and Ice Cream: Effect of Probiotic <i>Bifidobacterium</i> spp. on Survival of <i>Listeria monocytogenes</i> in Soft Cheese |
| title_sort | occurrence of i listeria i spp in soft cheese and ice cream effect of probiotic i bifidobacterium i spp on survival of i listeria monocytogenes i in soft cheese |
| topic | listeriosis probiotic <i>Bifidobacterium</i> dairy products preservatives inhibition |
| url | https://www.mdpi.com/2304-8158/11/21/3443 |
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