High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques
The demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltratio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/2/264 |
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| author | Felix M. Carbajal Gamarra José C. C. Santana Segundo A. V. Llanos Jorge A. Heredia Pérez Fábio Richard Flausino Ada P. B. Quispe Pedro Córdova Mendoza Rosangela M. Vanalle Carmen Carreño-Farfan Fernando T. Berssaneti Roberto R. de Souza Elias B. Tambourgi |
| author_facet | Felix M. Carbajal Gamarra José C. C. Santana Segundo A. V. Llanos Jorge A. Heredia Pérez Fábio Richard Flausino Ada P. B. Quispe Pedro Córdova Mendoza Rosangela M. Vanalle Carmen Carreño-Farfan Fernando T. Berssaneti Roberto R. de Souza Elias B. Tambourgi |
| author_sort | Felix M. Carbajal Gamarra |
| collection | DOAJ |
| description | The demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltration from pineapple juice (<i>Ananas comusus</i> L. Merrill). All assays were carried out at pH 7.0 and 7.5, and at 0.05 and 0.40 bar of transmembrane pressures. Results have shown that at the best operating conditions, between 85 and 87% of bromelain activity was recovered using the plain membrane separation process at 0.05 bar. The ultrafiltration has shown the capacity to retain 100% of proteolytic activity of the bromelain extracted. The samples have kept the same physics properties after ultrafiltration, and the result was verified via electrophoresis. The bromelain enzyme obtained was characterized, and pH 7 and between 30 and 40 °C were the best conditions. Therefore, this work shows that the use of both polymeric membranes has shown high efficiency, and can be used in the purification of bromelain enzymes. |
| first_indexed | 2024-03-10T00:40:14Z |
| format | Article |
| id | doaj.art-5904497fd33e43588d352b2dc5a82c1a |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T00:40:14Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-5904497fd33e43588d352b2dc5a82c1a2023-11-23T15:08:42ZengMDPI AGPolymers2073-43602022-01-0114226410.3390/polym14020264High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes TechniquesFelix M. Carbajal Gamarra0José C. C. Santana1Segundo A. V. Llanos2Jorge A. Heredia Pérez3Fábio Richard Flausino4Ada P. B. Quispe5Pedro Córdova Mendoza6Rosangela M. Vanalle7Carmen Carreño-Farfan8Fernando T. Berssaneti9Roberto R. de Souza10Elias B. Tambourgi11Energy Engineering, University of Brasilia, FGA-UnB, St. Leste Projeção A—Gama Leste, Brasilia 72444-240, DF, BrazilDepartment of Management Engineering, Federal University of ABC, University Mall, São Bernardo do Campo 09606-045, SP, BrazilFacultad de Ingeniería Química e Industrias Alimentarias, CYMAIDS, Universidad Nacional Pedro Ruiz Gallo, Calle Juan XXIII 391, Lambayeque 14013, PeruBusiness School, Universidad del Pacífico, Calle Sanchez Cerro 2141, Jesús Maria, Lima 15072, PeruIndustrial Engineering Postgraduate Program, Nine July University, Vergueiro Street, Liberdade, São Paulo 01504-001, SP, BrazilFacultad de Ingeniería Química e Industrias Alimentarias, CYMAIDS, Universidad Nacional Pedro Ruiz Gallo, Calle Juan XXIII 391, Lambayeque 14013, PeruFacultad de Ingeniería Ambiental y Sanitaria, Universidad Nacional San Luis Gonzaga de Ica, Ciudad Universitaria, Km 305, Ica 11004, PeruIndustrial Engineering Postgraduate Program, Nine July University, Vergueiro Street, Liberdade, São Paulo 01504-001, SP, BrazilFacultad de Ciencias Biológicas, CYMAIDS, Universidad Nacional Pedro Ruiz Gallo, Calle Juan XXIII 391, Lambayeque 14013, PeruDepartment of Production Engineering, Polytechnic School of State University of São Paulo, Av. Prof. Luciano Gualberto, 1380—Butantã, São Paulo 05508-010, SP, BrazilDepartment of Chemical Engineering, Federal University of Sergipe, DEQ/UFS, University Campus “José Aloísio de Campos”, Av. Marechal Rondon, S/N, Rosa Elze, São Cristóvão 49100-000, SP, BrazilSchool of Chemical Engineering, State University of Campinas, DESQ/FEQ/UNICAMP, University Campus “ZeferinoVaz”, Av. Albert Einstein, 500, Campinas 6066, São Paulo 13083-840, SP, BrazilThe demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltration from pineapple juice (<i>Ananas comusus</i> L. Merrill). All assays were carried out at pH 7.0 and 7.5, and at 0.05 and 0.40 bar of transmembrane pressures. Results have shown that at the best operating conditions, between 85 and 87% of bromelain activity was recovered using the plain membrane separation process at 0.05 bar. The ultrafiltration has shown the capacity to retain 100% of proteolytic activity of the bromelain extracted. The samples have kept the same physics properties after ultrafiltration, and the result was verified via electrophoresis. The bromelain enzyme obtained was characterized, and pH 7 and between 30 and 40 °C were the best conditions. Therefore, this work shows that the use of both polymeric membranes has shown high efficiency, and can be used in the purification of bromelain enzymes.https://www.mdpi.com/2073-4360/14/2/264pineapplebromelainseparation processhollow fiber membraneplain membraneenzymatic activity |
| spellingShingle | Felix M. Carbajal Gamarra José C. C. Santana Segundo A. V. Llanos Jorge A. Heredia Pérez Fábio Richard Flausino Ada P. B. Quispe Pedro Córdova Mendoza Rosangela M. Vanalle Carmen Carreño-Farfan Fernando T. Berssaneti Roberto R. de Souza Elias B. Tambourgi High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques Polymers pineapple bromelain separation process hollow fiber membrane plain membrane enzymatic activity |
| title | High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques |
| title_full | High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques |
| title_fullStr | High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques |
| title_full_unstemmed | High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques |
| title_short | High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques |
| title_sort | high retention and purification of bromelain enzyme i ananas comosus i l merrill from pineapple juice using plain and hollow polymeric membranes techniques |
| topic | pineapple bromelain separation process hollow fiber membrane plain membrane enzymatic activity |
| url | https://www.mdpi.com/2073-4360/14/2/264 |
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