Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes

Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes

Abstract Background Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus are major bacterial causes of lower respiratory tract infections (LRTIs) globally, leading to substantial morbidity and mortality. The rapi...

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Main Authors: Tran Thi Ngoc Dung, Voong Vinh Phat, Chau Vinh, Nguyen Phu Huong Lan, Nguyen Luong Nha Phuong, Le Thi Quynh Ngan, Guy Thwaites, Louise Thwaites, Maia Rabaa, Anh T. K. Nguyen, Pham Thanh Duy
Format: Article
Language:English
Published: BMC 2024-02-01
Series:BMC Infectious Diseases
Subjects:
Real-time PCR
Lower respiratory tract infections
Antimicrobial resistance
Melting curve analysis
Molecular diagnostics
Online Access:https://doi.org/10.1186/s12879-024-09028-2

Internet

https://doi.org/10.1186/s12879-024-09028-2

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