Protocol for the purification and transcriptomic analysis of mouse astrocytes using GFAT

Summary: Astrocytes are glial cells of the central nervous system that modulate neuronal function. Here, we present glyoxal-fixed astrocyte nuclei transcriptomics (GFAT), a protocol for the purification and transcriptomic analysis of astrocyte nuclei from the cortex and cerebellum of adult and aged...

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Bibliographic Details
Main Authors: Lara Labarta-Bajo, James Deng, Minerva Contreras, Nicola J. Allen
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672300566X