Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array
Stripe rust (caused by Puccinia striiformis f. sp. tritici) is one of the most severe diseases affecting wheat production. The disease is best controlled by developing and growing resistant cultivars. Chinese wheat (Triticum aestivum) landraces have excellent resistance to stripe rust. The objective...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.783830/full |
| _version_ | 1819320139357618176 |
|---|---|
| author | Fangjie Yao Fangnian Guan Luyao Duan Li Long Hao Tang Yunfeng Jiang Hao Li Qiantao Jiang Qiantao Jiang Jirui Wang Jirui Wang Pengfei Qi Houyang Kang Houyang Kang Wei Li Jian Ma Zhien Pu Mei Deng Yuming Wei Yuming Wei Youliang Zheng Youliang Zheng Xianming Chen Xianming Chen Guoyue Chen Guoyue Chen |
| author_facet | Fangjie Yao Fangnian Guan Luyao Duan Li Long Hao Tang Yunfeng Jiang Hao Li Qiantao Jiang Qiantao Jiang Jirui Wang Jirui Wang Pengfei Qi Houyang Kang Houyang Kang Wei Li Jian Ma Zhien Pu Mei Deng Yuming Wei Yuming Wei Youliang Zheng Youliang Zheng Xianming Chen Xianming Chen Guoyue Chen Guoyue Chen |
| author_sort | Fangjie Yao |
| collection | DOAJ |
| description | Stripe rust (caused by Puccinia striiformis f. sp. tritici) is one of the most severe diseases affecting wheat production. The disease is best controlled by developing and growing resistant cultivars. Chinese wheat (Triticum aestivum) landraces have excellent resistance to stripe rust. The objectives of this study were to identify wheat landraces with stable resistance and map quantitative trait loci (QTL) for resistance to stripe rust from 271 Chinese wheat landraces using a genome-wide association study (GWAS) approach. The landraces were phenotyped for stripe rust responses at the seedling stage with two predominant Chinese races of P. striiformis f. sp. tritici in a greenhouse and the adult-plant stage in four field environments and genotyped using the 660K wheat single-nucleotide polymorphism (SNP) array. Thirteen landraces with stable resistance were identified, and 17 QTL, including eight associated to all-stage resistance and nine to adult-plant resistance, were mapped on chromosomes 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 6D, and 7A. These QTL explained 6.06–16.46% of the phenotypic variation. Five of the QTL, QYrCL.sicau-3AL, QYrCL.sicau-3B.4, QYrCL.sicau-3B.5, QYrCL.sicau-5AL.1 and QYrCL.sicau-7AL, were likely new. Five Kompetitive allele specific PCR (KASP) markers for four of the QTL were converted from the significant SNP markers. The identified wheat landraces with stable resistance to stripe rust, significant QTL, and KASP markers should be useful for breeding wheat cultivars with durable resistance to stripe rust. |
| first_indexed | 2024-12-24T11:14:50Z |
| format | Article |
| id | doaj.art-5959610b275c43b4a4800c9e3368a26e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-24T11:14:50Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5959610b275c43b4a4800c9e3368a26e2022-12-21T16:58:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-12-011210.3389/fpls.2021.783830783830Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array Fangjie Yao0Fangnian Guan1Luyao Duan2Li Long3Hao Tang4Yunfeng Jiang5Hao Li6Qiantao Jiang7Qiantao Jiang8Jirui Wang9Jirui Wang10Pengfei Qi11Houyang Kang12Houyang Kang13Wei Li14Jian Ma15Zhien Pu16Mei Deng17Yuming Wei18Yuming Wei19Youliang Zheng20Youliang Zheng21Xianming Chen22Xianming Chen23Guoyue Chen24Guoyue Chen25Triticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaCollege of Agronomy, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaCollege of Agronomy, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaCollege of Agronomy, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaWheat Health, Genetics and Quality Research Unit, United States Department of Agriculture, Agricultural Research Service, Pullman, WA, United StatesDepartment of Plant Pathology, Washington State University, Pullman, WA, United StatesTriticeae Research Institute, Sichuan Agricultural University, Chengdu, ChinaState Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, ChinaStripe rust (caused by Puccinia striiformis f. sp. tritici) is one of the most severe diseases affecting wheat production. The disease is best controlled by developing and growing resistant cultivars. Chinese wheat (Triticum aestivum) landraces have excellent resistance to stripe rust. The objectives of this study were to identify wheat landraces with stable resistance and map quantitative trait loci (QTL) for resistance to stripe rust from 271 Chinese wheat landraces using a genome-wide association study (GWAS) approach. The landraces were phenotyped for stripe rust responses at the seedling stage with two predominant Chinese races of P. striiformis f. sp. tritici in a greenhouse and the adult-plant stage in four field environments and genotyped using the 660K wheat single-nucleotide polymorphism (SNP) array. Thirteen landraces with stable resistance were identified, and 17 QTL, including eight associated to all-stage resistance and nine to adult-plant resistance, were mapped on chromosomes 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 6D, and 7A. These QTL explained 6.06–16.46% of the phenotypic variation. Five of the QTL, QYrCL.sicau-3AL, QYrCL.sicau-3B.4, QYrCL.sicau-3B.5, QYrCL.sicau-5AL.1 and QYrCL.sicau-7AL, were likely new. Five Kompetitive allele specific PCR (KASP) markers for four of the QTL were converted from the significant SNP markers. The identified wheat landraces with stable resistance to stripe rust, significant QTL, and KASP markers should be useful for breeding wheat cultivars with durable resistance to stripe rust.https://www.frontiersin.org/articles/10.3389/fpls.2021.783830/fullwheat landracesresistancestripe rustGWASKASP markers |
| spellingShingle | Fangjie Yao Fangnian Guan Luyao Duan Li Long Hao Tang Yunfeng Jiang Hao Li Qiantao Jiang Qiantao Jiang Jirui Wang Jirui Wang Pengfei Qi Houyang Kang Houyang Kang Wei Li Jian Ma Zhien Pu Mei Deng Yuming Wei Yuming Wei Youliang Zheng Youliang Zheng Xianming Chen Xianming Chen Guoyue Chen Guoyue Chen Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array Frontiers in Plant Science wheat landraces resistance stripe rust GWAS KASP markers |
| title | Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array |
| title_full | Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array |
| title_fullStr | Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array |
| title_full_unstemmed | Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array |
| title_short | Genome-Wide Association Analysis of Stable Stripe Rust Resistance Loci in a Chinese Wheat Landrace Panel Using the 660K SNP Array |
| title_sort | genome wide association analysis of stable stripe rust resistance loci in a chinese wheat landrace panel using the 660k snp array |
| topic | wheat landraces resistance stripe rust GWAS KASP markers |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.783830/full |
| work_keys_str_mv | AT fangjieyao genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT fangnianguan genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT luyaoduan genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT lilong genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT haotang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT yunfengjiang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT haoli genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT qiantaojiang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT qiantaojiang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT jiruiwang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT jiruiwang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT pengfeiqi genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT houyangkang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT houyangkang genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT weili genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT jianma genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT zhienpu genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT meideng genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT yumingwei genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT yumingwei genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT youliangzheng genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT youliangzheng genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT xianmingchen genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT xianmingchen genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT guoyuechen genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray AT guoyuechen genomewideassociationanalysisofstablestriperustresistancelociinachinesewheatlandracepanelusingthe660ksnparray |