A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens

A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens

Abstract Mapping genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR interference (CRISPRi) based platform, compatible with Fluorescence Activated Cell Sorting (FACS)-based reporter screens, to query epistatic relat...

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Bibliographic Details
Main Authors: Alina Guna, Katharine R. Page, Joseph M. Replogle, Theodore K. Esantsi, Maxine L. Wang, Jonathan S. Weissman, Rebecca M. Voorhees
Format: Article
Language:English
Published: BMC 2023-10-01
Series:BMC Genomics
Subjects:
CRISPR interference
Genetic modifier
Epistasis
Genome-wide screen
ER membrane protein complex
Tail-anchored proteins
Online Access:https://doi.org/10.1186/s12864-023-09754-y

Internet

https://doi.org/10.1186/s12864-023-09754-y

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