Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)

Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)

Abstract Background The yeast Komagataella phaffii (Pichia pastoris) is routinely used for heterologous protein expression and is suggested as a model organism for yeast. Despite its importance and application potential, no reference gene for transcript analysis via RT-qPCR assays has been evaluated...

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Bibliographic Details
Main Authors: Mihail Besleaga, Gabriel A. Vignolle, Julian Kopp, Oliver Spadiut, Robert L. Mach, Astrid R. Mach-Aigner, Christian Zimmermann
Format: Article
Language:English
Published: BMC 2023-03-01
Series:Fungal Biology and Biotechnology
Subjects:
Komagataella phaffii
Pichia pastoris
RT-qPCR
Relative transcript analysis
Transcriptomics
Housekeeping genes
Online Access:https://doi.org/10.1186/s40694-023-00154-1

Internet

https://doi.org/10.1186/s40694-023-00154-1

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