High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing

High-throughput and high-accuracy single-cell RNA isoform analysis using PacBio circular consensus sequencing

Abstract Although long-read single-cell RNA isoform sequencing (scISO-Seq) can reveal alternative RNA splicing in individual cells, it suffers from a low read throughput. Here, we introduce HIT-scISOseq, a method that removes most artifact cDNAs and concatenates multiple cDNAs for PacBio circular co...

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Bibliographic Details
Main Authors: Zhuo-Xing Shi, Zhi-Chao Chen, Jia-Yong Zhong, Kun-Hua Hu, Ying-Feng Zheng, Ying Chen, Shang-Qian Xie, Xiao-Chen Bo, Feng Luo, Chong Tang, Chuan-Le Xiao, Yi-Zhi Liu
Format: Article
Language:English
Published: Nature Portfolio 2023-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-38324-9

Internet

https://doi.org/10.1038/s41467-023-38324-9

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