Auto-regulation of Rab5 GEF activity in Rabex5 by allosteric structural changes, catalytic core dynamics and ubiquitin binding

Auto-regulation of Rab5 GEF activity in Rabex5 by allosteric structural changes, catalytic core dynamics and ubiquitin binding

Intracellular trafficking depends on the function of Rab GTPases, whose activation is regulated by guanine exchange factors (GEFs). The Rab5 GEF, Rabex5, was previously proposed to be auto-inhibited by its C-terminus. Here, we studied full-length Rabex5 and Rabaptin5 proteins as well as domain delet...

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Bibliographic Details
Main Authors: Janelle Lauer, Sandra Segeletz, Alice Cezanne, Giambattista Guaitoli, Francesco Raimondi, Marc Gentzel, Vikram Alva, Michael Habeck, Yannis Kalaidzidis, Marius Ueffing, Andrei N Lupas, Christian Johannes Gloeckner, Marino Zerial
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-11-01
Series:eLife
Subjects:
hydrogen deuterium exchange mass spectrometry (HDX-MS)
guanine nucleotide exchange factor (GEF)
Rab5
Online Access:https://elifesciences.org/articles/46302

Internet

https://elifesciences.org/articles/46302

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