Analyzing efficiency of a lentiviral shRNA knockdown system in human enteroids using western blot and flow cytometry

Analyzing efficiency of a lentiviral shRNA knockdown system in human enteroids using western blot and flow cytometry

Summary: Enteroids are in vitro models to study gastrointestinal pathologies and test personalized therapeutics; however, the inherent complexity of enteroids often renders standard gene editing approaches ineffective. Here, we introduce a refined lentiviral transfection protocol, ensuring sufficien...

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Bibliographic Details
Main Authors: Adam P. Wilson, Karni S. Moshal, Addison P. Franca, Sasirekha Ramani, Randle Gallucci, Hala Chaaban, Kathryn Y. Burge
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
cell culture
flow cytometry
gene expression
protein expression and purification
cell differentiation
organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724002478

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http://www.sciencedirect.com/science/article/pii/S2666166724002478

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