Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury
Recently, adipose tissue-derived stem cells (ASCs) were emerged as an alternative, abundant, and easily accessible source of stem cell therapy. Previous studies revealed losartan (an angiotensin II type I receptor blocker) treatment promoted the healing of skeletal muscle by attenuation of the TGF-β...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-11-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X637055 |
| _version_ | 1828875943195705344 |
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| author | Jin-Kyu Park Mi-Ran Ki Eun-Mi Lee Ah-Young Kim Sang-Young You Seon-Young Han Eun-Joo Lee Il-Hwa Hong Soon-Hak Kwon Seong-Jin Kim Thomas A. Rando Kyu-Shik Jeong D.V.M., Ph.D. |
| author_facet | Jin-Kyu Park Mi-Ran Ki Eun-Mi Lee Ah-Young Kim Sang-Young You Seon-Young Han Eun-Joo Lee Il-Hwa Hong Soon-Hak Kwon Seong-Jin Kim Thomas A. Rando Kyu-Shik Jeong D.V.M., Ph.D. |
| author_sort | Jin-Kyu Park |
| collection | DOAJ |
| description | Recently, adipose tissue-derived stem cells (ASCs) were emerged as an alternative, abundant, and easily accessible source of stem cell therapy. Previous studies revealed losartan (an angiotensin II type I receptor blocker) treatment promoted the healing of skeletal muscle by attenuation of the TGF-β signaling pathway, which inhibits muscle differentiation. Therefore, we hypothesized that a combined therapy using ASCs and losartan might dramatically improve the muscle remodeling after muscle injury. To determine the combined effect of losartan with ASC transplantation, we created a muscle laceration mouse model. EGFP-labeled ASCs were locally transplanted to the injured gastrocnemius muscle after muscle laceration. The dramatic muscle regeneration and the remarkably inhibited muscular fibrosis were observed by combined treatment. Transplanted ASCs fused with the injured or differentiating myofibers. Myotube formation was also enhanced by ASC + satellite coculture and losartan treatment. Thus, the present study indicated that ASC transplantation effect for skeletal muscle injury can be dramatically improved by losartan treatment inducing better niche. |
| first_indexed | 2024-12-13T08:10:04Z |
| format | Article |
| id | doaj.art-59c824f2c14c4a1e9a628f8591c1a24c |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T08:10:04Z |
| publishDate | 2012-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-59c824f2c14c4a1e9a628f8591c1a24c2022-12-21T23:54:14ZengSAGE PublishingCell Transplantation0963-68971555-38922012-11-012110.3727/096368912X637055Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle InjuryJin-Kyu Park0Mi-Ran Ki1Eun-Mi Lee2Ah-Young Kim3Sang-Young You4Seon-Young Han5Eun-Joo Lee6Il-Hwa Hong7Soon-Hak Kwon8Seong-Jin Kim9Thomas A. Rando10Kyu-Shik Jeong D.V.M., Ph.D.11College of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, South KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, South KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, South KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, South KoreaStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, South KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Pediatrics, School of Medicine, Kyungpook National University, Daegu, South KoreaCHA Cancer Institute, CHA University, Seoul, South KoreaDepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USAStem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, South KoreaRecently, adipose tissue-derived stem cells (ASCs) were emerged as an alternative, abundant, and easily accessible source of stem cell therapy. Previous studies revealed losartan (an angiotensin II type I receptor blocker) treatment promoted the healing of skeletal muscle by attenuation of the TGF-β signaling pathway, which inhibits muscle differentiation. Therefore, we hypothesized that a combined therapy using ASCs and losartan might dramatically improve the muscle remodeling after muscle injury. To determine the combined effect of losartan with ASC transplantation, we created a muscle laceration mouse model. EGFP-labeled ASCs were locally transplanted to the injured gastrocnemius muscle after muscle laceration. The dramatic muscle regeneration and the remarkably inhibited muscular fibrosis were observed by combined treatment. Transplanted ASCs fused with the injured or differentiating myofibers. Myotube formation was also enhanced by ASC + satellite coculture and losartan treatment. Thus, the present study indicated that ASC transplantation effect for skeletal muscle injury can be dramatically improved by losartan treatment inducing better niche.https://doi.org/10.3727/096368912X637055 |
| spellingShingle | Jin-Kyu Park Mi-Ran Ki Eun-Mi Lee Ah-Young Kim Sang-Young You Seon-Young Han Eun-Joo Lee Il-Hwa Hong Soon-Hak Kwon Seong-Jin Kim Thomas A. Rando Kyu-Shik Jeong D.V.M., Ph.D. Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury Cell Transplantation |
| title | Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury |
| title_full | Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury |
| title_fullStr | Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury |
| title_full_unstemmed | Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury |
| title_short | Losartan Improves Adipose Tissue-Derived Stem Cell Niche by Inhibiting Transforming Growth Factor-β and Fibrosis in Skeletal Muscle Injury |
| title_sort | losartan improves adipose tissue derived stem cell niche by inhibiting transforming growth factor β and fibrosis in skeletal muscle injury |
| url | https://doi.org/10.3727/096368912X637055 |
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