A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro

A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro

IntroductionGlass coverslips are used as a substrate since Harrison’s initial nerve cell culture experiments in 1910. In 1974, the first study of brain cells seeded onto polylysine (PL) coated substrate was published. Usually, neurons adhere quickly to PL coating. However, maintaining cortical neuro...

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Bibliographic Details
Main Authors: Aurélie Stil, Benoît Liberelle, Dainelys Guadarrama Bello, Lucile Lacomme, Laurie Arpin, Pascale Parent, Antonio Nanci, Éric C. Dumont, Tarek Ould-Bachir, Matthieu P. Vanni, Gregory De Crescenzo, Jean-François Bouchard
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Cellular Neuroscience
Subjects:
primary cortical neuron cultures
glass coverslip
poly-D-lysine grafting
neuronal adhesion
neuronal maturation
synaptic contacts
Online Access:https://www.frontiersin.org/articles/10.3389/fncel.2023.1212097/full

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https://www.frontiersin.org/articles/10.3389/fncel.2023.1212097/full

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