DEVELOPMENT OF A MULTIPLEX ALLELE-SPECIFIC REAL-TIME PCR METHOD FOR DETECTION OF PIK3CA GENE SOMATIC MUTATIONS AND ITS VALIDATION IN THE TUMORS OF BREAST CANCER PATIENTS

DEVELOPMENT OF A MULTIPLEX ALLELE-SPECIFIC REAL-TIME PCR METHOD FOR DETECTION OF PIK3CA GENE SOMATIC MUTATIONS AND ITS VALIDATION IN THE TUMORS OF BREAST CANCER PATIENTS

Aim: To develop a highly sensitive real-time polymerase chain reaction (PCR) system for detection of somatic mutations in 542 and 545 codons of exon 9 and 1047 codon of exon 20 of PIK3CA gene comprising more than 80% of all somatic mutations in this gene for application on histological material with...

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Bibliographic Details
Main Authors: M. L. Filipenko, D. V. Shamovskaya, N. A. Oskina, I. P. Oscorbin, E. A. Khrapov, L. K. Ovchinnikova, E. S. Gershteyn, N. E. Kushlinskii
Format: Article
Language:Russian
Published: MONIKI 2016-02-01
Series:Alʹmanah Kliničeskoj Mediciny
Subjects:
allele-specific real-time pcr (as-rtpcr)
pik3ca gene
mutations
breast cancer
Online Access:https://www.almclinmed.ru/jour/article/view/70

Internet

https://www.almclinmed.ru/jour/article/view/70

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