The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.
The human protein ASPG is an enzyme with a putative antitumor activity. We generated in bacteria and then purified a recombinant GST-ASPG protein that we used to characterize the biochemical and cytotoxic properties of the human ASPG. We demonstrated that ASPG possesses asparaginase and PAF acetylhy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5443537?pdf=render |
| _version_ | 1818478326617997312 |
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| author | Stefania Belviso Rodolfo Iuliano Rosario Amato Nicola Perrotti Miranda Menniti |
| author_facet | Stefania Belviso Rodolfo Iuliano Rosario Amato Nicola Perrotti Miranda Menniti |
| author_sort | Stefania Belviso |
| collection | DOAJ |
| description | The human protein ASPG is an enzyme with a putative antitumor activity. We generated in bacteria and then purified a recombinant GST-ASPG protein that we used to characterize the biochemical and cytotoxic properties of the human ASPG. We demonstrated that ASPG possesses asparaginase and PAF acetylhydrolase activities that depend on a critical threonine residue at position 19. Consistently, ASPG but not its T19A mutant showed cytotoxic activity in K562, NALM-6 and MOLT-4 leukemic cell lines but not in normal cells. Regarding the mechanism of action of ASPG, it was able to induce a significant apoptotic death in K562 cells. Taken together our data suggest that ASPG, combining different enzymatic activities, should be considered a promising anti-cancer agent for inhibiting the growth of leukemia cells. |
| first_indexed | 2024-12-10T09:46:38Z |
| format | Article |
| id | doaj.art-5a0ea9f2ddd24f489382def7cdedae1e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:46:38Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5a0ea9f2ddd24f489382def7cdedae1e2022-12-22T01:53:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017817410.1371/journal.pone.0178174The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.Stefania BelvisoRodolfo IulianoRosario AmatoNicola PerrottiMiranda MennitiThe human protein ASPG is an enzyme with a putative antitumor activity. We generated in bacteria and then purified a recombinant GST-ASPG protein that we used to characterize the biochemical and cytotoxic properties of the human ASPG. We demonstrated that ASPG possesses asparaginase and PAF acetylhydrolase activities that depend on a critical threonine residue at position 19. Consistently, ASPG but not its T19A mutant showed cytotoxic activity in K562, NALM-6 and MOLT-4 leukemic cell lines but not in normal cells. Regarding the mechanism of action of ASPG, it was able to induce a significant apoptotic death in K562 cells. Taken together our data suggest that ASPG, combining different enzymatic activities, should be considered a promising anti-cancer agent for inhibiting the growth of leukemia cells.http://europepmc.org/articles/PMC5443537?pdf=render |
| spellingShingle | Stefania Belviso Rodolfo Iuliano Rosario Amato Nicola Perrotti Miranda Menniti The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells. PLoS ONE |
| title | The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells. |
| title_full | The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells. |
| title_fullStr | The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells. |
| title_full_unstemmed | The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells. |
| title_short | The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells. |
| title_sort | human asparaginase enzyme aspg inhibits growth in leukemic cells |
| url | http://europepmc.org/articles/PMC5443537?pdf=render |
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