Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale

Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale

The CRISPR/Cas9 system is extensively used for plant gene editing. This study developed an efficient CRISPR/Cas9 system for Chinese kale using multiple sgRNAs and two promoters to create various CRISPR/Cas9 vectors. These vectors targeted <i>BoaZDS</i> and <i>BoaCRTISO</i> in...

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Bibliographic Details
Main Authors: Wenli Huang, Aihong Zheng, Huanhuan Huang, Zhifeng Chen, Jie Ma, Xiangxiang Li, Qiannan Liang, Ling Li, Ruobin Liu, Zhi Huang, Yaoguo Qin, Yi Tang, Huanxiu Li, Fen Zhang, Qiaomei Wang, Bo Sun
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:International Journal of Molecular Sciences
Subjects:
Chinese kale
CRISPR/Cas9
gene editing
sgRNAs
vectors
explants
Online Access:https://www.mdpi.com/1422-0067/24/17/13241

Internet

https://www.mdpi.com/1422-0067/24/17/13241

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