Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing

Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing

ABSTRACT Downstream next-generation sequencing (NGS) of the syphilis spirochete Treponema pallidum subspecies pallidum (T. pallidum) is hindered by low bacterial loads and the overwhelming presence of background metagenomic DNA in clinical specimens. In this study, we investigated selective whole-ge...

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Bibliographic Details
Main Authors: Charles M. Thurlow, Sandeep J. Joseph, Lilia Ganova-Raeva, Samantha S. Katz, Lara Pereira, Cheng Chen, Alyssa Debra, Kendra Vilfort, Kimberly Workowski, Stephanie E. Cohen, Hilary Reno, Yongcheng Sun, Mark Burroughs, Mili Sheth, Kai-Hua Chi, Damien Danavall, Susan S. Philip, Weiping Cao, Ellen N. Kersh, Allan Pillay
Format: Article
Language:English
Published: American Society for Microbiology 2022-06-01
Series:mSphere
Subjects:
DNA enrichment
Treponema pallidum
metagenomics
syphilis
whole-genome sequencing
Online Access:https://journals.asm.org/doi/10.1128/msphere.00009-22

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https://journals.asm.org/doi/10.1128/msphere.00009-22

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