Cryopreservation of spin-dried mammalian cells.

This study reports an alternative approach to achieve vitrification where cells are pre-desiccated prior to cooling to cryogenic temperatures for storage. Chinese Hamster Ovary (CHO) cells suspended in a trehalose solution were rapidly and uniformly desiccated to a low moisture content (<0.12 g o...

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Bibliographic Details
Main Authors: Nilay Chakraborty, Michael A Menze, Jason Malsam, Alptekin Aksan, Steven C Hand, Mehmet Toner
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3178566?pdf=render