The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection
Abstract Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. Howe...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-04-01
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| Series: | Protein & Cell |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007/s13238-017-0405-7 |
| _version_ | 1827842451074711552 |
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| author | Xuyuan Zhang Pan Yang Nan Wang Jialong Zhang Jingyun Li Hao Guo Xiangyun Yin Zihe Rao Xiangxi Wang Liguo Zhang |
| author_facet | Xuyuan Zhang Pan Yang Nan Wang Jialong Zhang Jingyun Li Hao Guo Xiangyun Yin Zihe Rao Xiangxi Wang Liguo Zhang |
| author_sort | Xuyuan Zhang |
| collection | DOAJ |
| description | Abstract Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77–113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry. |
| first_indexed | 2024-03-12T08:09:43Z |
| format | Article |
| id | doaj.art-5a97ebda52f245c29766143393ddccd3 |
| institution | Directory Open Access Journal |
| issn | 1674-800X 1674-8018 |
| language | English |
| last_indexed | 2024-03-12T08:09:43Z |
| publishDate | 2017-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Protein & Cell |
| spelling | doaj.art-5a97ebda52f245c29766143393ddccd32023-09-02T19:12:25ZengOxford University PressProtein & Cell1674-800X1674-80182017-04-018859060010.1007/s13238-017-0405-7The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infectionXuyuan Zhang0Pan Yang1Nan Wang2Jialong Zhang3Jingyun Li4Hao Guo5Xiangyun Yin6Zihe Rao7Xiangxi Wang8Liguo Zhang9Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesKey Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of SciencesKey Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of SciencesKey Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of SciencesKey Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesKey Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of SciencesAbstract Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77–113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.http://link.springer.com/article/10.1007/s13238-017-0405-7SCARB2EV71monoclonal antibodyHFMDreceptor |
| spellingShingle | Xuyuan Zhang Pan Yang Nan Wang Jialong Zhang Jingyun Li Hao Guo Xiangyun Yin Zihe Rao Xiangxi Wang Liguo Zhang The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection Protein & Cell SCARB2 EV71 monoclonal antibody HFMD receptor |
| title | The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection |
| title_full | The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection |
| title_fullStr | The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection |
| title_full_unstemmed | The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection |
| title_short | The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection |
| title_sort | binding of a monoclonal antibody to the apical region of scarb2 blocks ev71 infection |
| topic | SCARB2 EV71 monoclonal antibody HFMD receptor |
| url | http://link.springer.com/article/10.1007/s13238-017-0405-7 |
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