Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC were compared with visual scoring results obtaine...

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Bibliographic Details
Main Authors: Maxime François, Kevin Hochstenbach, Wayne Leifert, Michael Felix Fenech
Format: Article
Language:English
Published: Taylor & Francis Group 2014-12-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114239