Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium
Fibroblasts have been shown to be one of the essential players for mammary gland organization. Here, we identify two major types of mouse mammary gland fibroblasts through single-cell RNA sequencing analysis: Dpp4+ fibroblasts and Dpp4- fibroblasts. Each population exhibits unique functional charact...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.850568/full |
| _version_ | 1819005971451609088 |
|---|---|
| author | Ryohei Yoshitake Gregory Chang Kohei Saeki Kohei Saeki Desiree Ha Xiwei Wu Jinhui Wang Shiuan Chen |
| author_facet | Ryohei Yoshitake Gregory Chang Kohei Saeki Kohei Saeki Desiree Ha Xiwei Wu Jinhui Wang Shiuan Chen |
| author_sort | Ryohei Yoshitake |
| collection | DOAJ |
| description | Fibroblasts have been shown to be one of the essential players for mammary gland organization. Here, we identify two major types of mouse mammary gland fibroblasts through single-cell RNA sequencing analysis: Dpp4+ fibroblasts and Dpp4- fibroblasts. Each population exhibits unique functional characteristics as well as discrete localization in normal mouse mammary glands. Remarkably, estrogen, a crucial mediator of mammary gland organization, alters the gene expression profiles of fibroblasts in a population-specific manner, without distinct activation of estrogen receptor signaling. Further integrative analysis with the inclusion of five other publicly available datasets reveals a directional differentiation among the mammary gland fibroblast populations. Moreover, the combination with the mouse mammary epithelium atlas allows us to infer multiple potential interactions between epithelial cells and fibroblasts in mammary glands. This study provides a comprehensive view of mouse mammary gland fibroblasts at the single-cell level. |
| first_indexed | 2024-12-21T00:01:16Z |
| format | Article |
| id | doaj.art-5a9c9344b700420bad7020a24bf486d0 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-21T00:01:16Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-5a9c9344b700420bad7020a24bf486d02022-12-21T19:22:36ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-03-011010.3389/fcell.2022.850568850568Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With EpitheliumRyohei Yoshitake0Gregory Chang1Kohei Saeki2Kohei Saeki3Desiree Ha4Xiwei Wu5Jinhui Wang6Shiuan Chen7Department of Cancer Biology, Beckman Research Institute of City of Hope, Duarte, CA, United StatesDepartment of Cancer Biology, Beckman Research Institute of City of Hope, Duarte, CA, United StatesDepartment of Cancer Biology, Beckman Research Institute of City of Hope, Duarte, CA, United StatesFaculty of Veterinary Medicine, Okayama University of Science, Imabari, JapanDepartment of Cancer Biology, Beckman Research Institute of City of Hope, Duarte, CA, United StatesIntegrative Genomics Core, Beckman Research Institute of City of Hope, Monrovia, CA, United StatesIntegrative Genomics Core, Beckman Research Institute of City of Hope, Monrovia, CA, United StatesDepartment of Cancer Biology, Beckman Research Institute of City of Hope, Duarte, CA, United StatesFibroblasts have been shown to be one of the essential players for mammary gland organization. Here, we identify two major types of mouse mammary gland fibroblasts through single-cell RNA sequencing analysis: Dpp4+ fibroblasts and Dpp4- fibroblasts. Each population exhibits unique functional characteristics as well as discrete localization in normal mouse mammary glands. Remarkably, estrogen, a crucial mediator of mammary gland organization, alters the gene expression profiles of fibroblasts in a population-specific manner, without distinct activation of estrogen receptor signaling. Further integrative analysis with the inclusion of five other publicly available datasets reveals a directional differentiation among the mammary gland fibroblast populations. Moreover, the combination with the mouse mammary epithelium atlas allows us to infer multiple potential interactions between epithelial cells and fibroblasts in mammary glands. This study provides a comprehensive view of mouse mammary gland fibroblasts at the single-cell level.https://www.frontiersin.org/articles/10.3389/fcell.2022.850568/fullcell-cell interactionestrogenfibroblastsmammary glandsingle-cell RNA sequencingtrajectory analysis |
| spellingShingle | Ryohei Yoshitake Gregory Chang Kohei Saeki Kohei Saeki Desiree Ha Xiwei Wu Jinhui Wang Shiuan Chen Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium Frontiers in Cell and Developmental Biology cell-cell interaction estrogen fibroblasts mammary gland single-cell RNA sequencing trajectory analysis |
| title | Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium |
| title_full | Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium |
| title_fullStr | Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium |
| title_full_unstemmed | Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium |
| title_short | Single-Cell Transcriptomics Identifies Heterogeneity of Mouse Mammary Gland Fibroblasts With Distinct Functions, Estrogen Responses, Differentiation Processes, and Crosstalks With Epithelium |
| title_sort | single cell transcriptomics identifies heterogeneity of mouse mammary gland fibroblasts with distinct functions estrogen responses differentiation processes and crosstalks with epithelium |
| topic | cell-cell interaction estrogen fibroblasts mammary gland single-cell RNA sequencing trajectory analysis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.850568/full |
| work_keys_str_mv | AT ryoheiyoshitake singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT gregorychang singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT koheisaeki singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT koheisaeki singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT desireeha singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT xiweiwu singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT jinhuiwang singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium AT shiuanchen singlecelltranscriptomicsidentifiesheterogeneityofmousemammaryglandfibroblastswithdistinctfunctionsestrogenresponsesdifferentiationprocessesandcrosstalkswithepithelium |