Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation
The effect of low-intensity sonication treatment on cell growth, <i>ε</i>-polylysine (<i>ε</i>-PL) yield and its biological mechanism were investigated, using a 3-L-jar fermenter coupled with an in situ ultrasonic slot with a <i>Streptomyces albulus</i> strain SAR...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/21/3525 |
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| author | Jiahui Xiang Mokhtar Dabbour Xianli Gao Benjamin Kumah Mintah Yao Yang Wenbin Ren Ronghai He Chunhua Dai Haile Ma |
| author_facet | Jiahui Xiang Mokhtar Dabbour Xianli Gao Benjamin Kumah Mintah Yao Yang Wenbin Ren Ronghai He Chunhua Dai Haile Ma |
| author_sort | Jiahui Xiang |
| collection | DOAJ |
| description | The effect of low-intensity sonication treatment on cell growth, <i>ε</i>-polylysine (<i>ε</i>-PL) yield and its biological mechanism were investigated, using a 3-L-jar fermenter coupled with an in situ ultrasonic slot with a <i>Streptomyces albulus</i> strain SAR 14-116. Under ultrasonic conditions (28 kHz, 0.37 W cm<sup>−2</sup>, 60 min), a high biomass of SAR 14-116 and concentration of <i>ε</i>-PL were realized (i.e., they increased by 14.92% and 28.45%, respectively) when compared with a control. Besides this, ultrasonication increased the mycelia viability and intracellular ATP as well as activities of key enzymes involved in the <i>ε</i>-PL biosynthesis pathway, resulting in an improvement in the production of <i>ε</i>-PL. Data on qRT-PCR revealed that ultrasonication also affected the gene expression of key enzymes in the <i>ε</i>-PL biosynthesis pathway, including <i>ε</i>-PL synthetase (PLS). These outcomes provided the basis for understanding the effects of ultrasound-assisted fermentation on the stimulation of metabolite production and fermentation procedure in a fermenter. |
| first_indexed | 2024-03-09T19:04:27Z |
| format | Article |
| id | doaj.art-5aa29b617f8949c1b7945822b6300574 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:04:27Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-5aa29b617f8949c1b7945822b63005742023-11-24T04:42:13ZengMDPI AGFoods2304-81582022-11-011121352510.3390/foods11213525Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> FermentationJiahui Xiang0Mokhtar Dabbour1Xianli Gao2Benjamin Kumah Mintah3Yao Yang4Wenbin Ren5Ronghai He6Chunhua Dai7Haile Ma8School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaDepartment of Agricultural and Biosystems Engineering, Faculty of Agriculture, Benha University, Qaluobia P.O. Box 13736, EgyptSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaCouncil for Scientific and Industrial Research—Food Research Institute, Accra P.O. Box M20, GhanaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, ChinaThe effect of low-intensity sonication treatment on cell growth, <i>ε</i>-polylysine (<i>ε</i>-PL) yield and its biological mechanism were investigated, using a 3-L-jar fermenter coupled with an in situ ultrasonic slot with a <i>Streptomyces albulus</i> strain SAR 14-116. Under ultrasonic conditions (28 kHz, 0.37 W cm<sup>−2</sup>, 60 min), a high biomass of SAR 14-116 and concentration of <i>ε</i>-PL were realized (i.e., they increased by 14.92% and 28.45%, respectively) when compared with a control. Besides this, ultrasonication increased the mycelia viability and intracellular ATP as well as activities of key enzymes involved in the <i>ε</i>-PL biosynthesis pathway, resulting in an improvement in the production of <i>ε</i>-PL. Data on qRT-PCR revealed that ultrasonication also affected the gene expression of key enzymes in the <i>ε</i>-PL biosynthesis pathway, including <i>ε</i>-PL synthetase (PLS). These outcomes provided the basis for understanding the effects of ultrasound-assisted fermentation on the stimulation of metabolite production and fermentation procedure in a fermenter.https://www.mdpi.com/2304-8158/11/21/3525<i>Streptomyces albulus</i><i>ε</i>-polylysineultrasound-assisted fermentationintracellular ATP<i>Biosynthesis</i> key enzymes |
| spellingShingle | Jiahui Xiang Mokhtar Dabbour Xianli Gao Benjamin Kumah Mintah Yao Yang Wenbin Ren Ronghai He Chunhua Dai Haile Ma Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation Foods <i>Streptomyces albulus</i> <i>ε</i>-polylysine ultrasound-assisted fermentation intracellular ATP <i>Biosynthesis</i> key enzymes |
| title | Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation |
| title_full | Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation |
| title_fullStr | Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation |
| title_full_unstemmed | Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation |
| title_short | Influence of Low-Intensity Ultrasound on <i>ε</i>-Polylysine Production: Intracellular ATP and Key Biosynthesis Enzymes during <i>Streptomyces albulus</i> Fermentation |
| title_sort | influence of low intensity ultrasound on i ε i polylysine production intracellular atp and key biosynthesis enzymes during i streptomyces albulus i fermentation |
| topic | <i>Streptomyces albulus</i> <i>ε</i>-polylysine ultrasound-assisted fermentation intracellular ATP <i>Biosynthesis</i> key enzymes |
| url | https://www.mdpi.com/2304-8158/11/21/3525 |
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