Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons

Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons

ABSTRACT Transposon mutagenesis with next-generation sequencing (TnSeq) is a powerful approach to annotate gene function in bacteria, but existing protocols for TnSeq require laborious preparation of every sample before sequencing. Thus, the existing protocols are not amenable to the throughput nece...

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Bibliographic Details
Main Authors: Kelly M. Wetmore, Morgan N. Price, Robert J. Waters, Jacob S. Lamson, Jennifer He, Cindi A. Hoover, Matthew J. Blow, James Bristow, Gareth Butland, Adam P. Arkin, Adam Deutschbauer
Format: Article
Language:English
Published: American Society for Microbiology 2015-07-01
Series:mBio
Online Access:https://journals.asm.org/doi/10.1128/mBio.00306-15

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https://journals.asm.org/doi/10.1128/mBio.00306-15

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