Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis
Objective: To evaluate the effect of hydroxysafflor yellow A (HSYA) on thioacetamide-induced liver fibrosis. Methods: Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks. Thioacetamide-intoxicated rats were given silymarin (50 mg/kg) or HSYA (5 mg...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=8;spage=348;epage=358;aulast=el-Din |
| _version_ | 1797649566782193664 |
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| author | Sayed H. Seif el-Din Olfat A. Hammam Shahira M. Ezzat Samira Saleh Marwa M. Safar Walaa H. El-Maadawy Naglaa M. El-Lakkany |
| author_facet | Sayed H. Seif el-Din Olfat A. Hammam Shahira M. Ezzat Samira Saleh Marwa M. Safar Walaa H. El-Maadawy Naglaa M. El-Lakkany |
| author_sort | Sayed H. Seif el-Din |
| collection | DOAJ |
| description | Objective: To evaluate the effect of hydroxysafflor yellow A (HSYA) on thioacetamide-induced liver fibrosis.
Methods: Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks. Thioacetamide-intoxicated rats were given silymarin (50 mg/kg) or HSYA (5 mg/kg) orally every day for 8 weeks. Liver enzymes, fibrosis markers, histological changes as well as immunohistochemistry of TNF-α, IL-6, p21, α-SMA, and caspase-3 were examined. The effect of HSYA on HSC-T6 activation/proliferation and apoptosis was also determined in vitro.
Results: HSYA decreased liver enzymes, TNF-α, IL-6, and p21 expressions, hepatic PDGF-B, TIMP-1, TGF-β1, and hydroxyproline levels, as well as fibrosis score (S2 vs. S4) compared to the thioacetamide group. HSYA also downregulated α-SMA while increasing caspase-3 expression. Surprisingly, at 500 μg/mL, HSYA had only a slightly suppressive effect on HSC proliferation, with a 9.5% reduction. However, it significantly reduced TGF-β1, inhibited α-SMA expression, induced caspase-3 expression, and promoted cell senescence.
Conclusions: HSYA may be a potential therapeutic agent for delaying and reversing the progression of liver fibrosis. More research on HSYA at higher doses and for a longer period is warranted. |
| first_indexed | 2024-03-11T15:48:56Z |
| format | Article |
| id | doaj.art-5ab834bfb0f14d9eba207c65580cd984 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-03-11T15:48:56Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-5ab834bfb0f14d9eba207c65580cd9842023-10-26T05:32:36ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222023-01-0113834835810.4103/2221-1691.383689Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosisSayed H. Seif el-DinOlfat A. HammamShahira M. EzzatSamira SalehMarwa M. SafarWalaa H. El-MaadawyNaglaa M. El-LakkanyObjective: To evaluate the effect of hydroxysafflor yellow A (HSYA) on thioacetamide-induced liver fibrosis. Methods: Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks. Thioacetamide-intoxicated rats were given silymarin (50 mg/kg) or HSYA (5 mg/kg) orally every day for 8 weeks. Liver enzymes, fibrosis markers, histological changes as well as immunohistochemistry of TNF-α, IL-6, p21, α-SMA, and caspase-3 were examined. The effect of HSYA on HSC-T6 activation/proliferation and apoptosis was also determined in vitro. Results: HSYA decreased liver enzymes, TNF-α, IL-6, and p21 expressions, hepatic PDGF-B, TIMP-1, TGF-β1, and hydroxyproline levels, as well as fibrosis score (S2 vs. S4) compared to the thioacetamide group. HSYA also downregulated α-SMA while increasing caspase-3 expression. Surprisingly, at 500 μg/mL, HSYA had only a slightly suppressive effect on HSC proliferation, with a 9.5% reduction. However, it significantly reduced TGF-β1, inhibited α-SMA expression, induced caspase-3 expression, and promoted cell senescence. Conclusions: HSYA may be a potential therapeutic agent for delaying and reversing the progression of liver fibrosis. More research on HSYA at higher doses and for a longer period is warranted.http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=8;spage=348;epage=358;aulast=el-Dinhydroxysafflor yellow a; thioacetamide; hepatic stellate cells; inflammatory markers; liver fibrosis; p21; α-sma apoptosis |
| spellingShingle | Sayed H. Seif el-Din Olfat A. Hammam Shahira M. Ezzat Samira Saleh Marwa M. Safar Walaa H. El-Maadawy Naglaa M. El-Lakkany Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis Asian Pacific Journal of Tropical Biomedicine hydroxysafflor yellow a; thioacetamide; hepatic stellate cells; inflammatory markers; liver fibrosis; p21; α-sma apoptosis |
| title | Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis |
| title_full | Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis |
| title_fullStr | Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis |
| title_full_unstemmed | Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis |
| title_short | Hydroxysafflor yellow A protects against thioacetamide-induced liver fibrosis in rats via suppressing proinflammatory/fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis |
| title_sort | hydroxysafflor yellow a protects against thioacetamide induced liver fibrosis in rats via suppressing proinflammatory fibrogenic mediators and promoting hepatic stellate cell senescence and apoptosis |
| topic | hydroxysafflor yellow a; thioacetamide; hepatic stellate cells; inflammatory markers; liver fibrosis; p21; α-sma apoptosis |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=8;spage=348;epage=358;aulast=el-Din |
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