Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Summary: Synthetic protocols providing mechanical patterns to culture substrate are essential to control the self-condensation of cells for organoid engineering. Here, we present a protocol for preparing hydrogels with mechanical patterns. We describe steps for hydrogel synthesis, mechanical evaluat...

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Bibliographic Details
Main Authors: Takahisa Matsuzaki, Yuma Kawano, Momoka Horikiri, Yuko Shimokawa, Takashi Yamazaki, Nao Okuma, Hiroyuki Koike, Masaki Kimura, Ryuzo Kawamura, Yosuke Yoneyama, Yasuro Furuichi, Fumihiko Hakuno, Shin-Ichiro Takahashi, Seiichiro Nakabayashi, Satoshi Okamoto, Hiromitsu Nakauchi, Hideki Taniguchi, Takanori Takebe, Hiroshi Y. Yoshikawa
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Biophysics
Atomic Force Microscopy (AFM)
Health Sciences
Stem Cells
Organoids
Tissue Engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004380

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http://www.sciencedirect.com/science/article/pii/S2666166723004380

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