Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

Summary: Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent o...

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Main Authors: Ishan Agrawal, Nidhi Sharma, Shivanjali Saxena, S. Arvind, Debayani Chakraborty, Debarati Bhunia Chakraborty, Deepak Jha, Surajit Ghatak, Sridhar Epari, Tejpal Gupta, Sushmita Jha
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003853