Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells
Summary: Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent o...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721003853 |
| _version_ | 1818663621542019072 |
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| author | Ishan Agrawal Nidhi Sharma Shivanjali Saxena S. Arvind Debayani Chakraborty Debarati Bhunia Chakraborty Deepak Jha Surajit Ghatak Sridhar Epari Tejpal Gupta Sushmita Jha |
| author_facet | Ishan Agrawal Nidhi Sharma Shivanjali Saxena S. Arvind Debayani Chakraborty Debarati Bhunia Chakraborty Deepak Jha Surajit Ghatak Sridhar Epari Tejpal Gupta Sushmita Jha |
| author_sort | Ishan Agrawal |
| collection | DOAJ |
| description | Summary: Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines.For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021). |
| first_indexed | 2024-12-17T05:19:46Z |
| format | Article |
| id | doaj.art-5ad1eeadfff146f6976b0e1d117b1dbb |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T05:19:46Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-5ad1eeadfff146f6976b0e1d117b1dbb2022-12-21T22:02:00ZengElsevierSTAR Protocols2666-16672021-09-0123100678Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cellsIshan Agrawal0Nidhi Sharma1Shivanjali Saxena2S. Arvind3Debayani Chakraborty4Debarati Bhunia Chakraborty5Deepak Jha6Surajit Ghatak7Sridhar Epari8Tejpal Gupta9Sushmita Jha10Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India; Corresponding authorDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India; Karolinska Institute and Scilifelab, Stockholm, SwedenDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, IndiaDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, IndiaDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, IndiaDepartment of Computer Science and Engineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, IndiaDepartment of Neurosurgery, All India Institute of Medical Sciences Jodhpur, Jodhpur, Rajasthan 342005, IndiaDepartment of Anatomy, All India Institute of Medical Sciences Jodhpur, Jodhpur, Rajasthan 342005, IndiaDepartment of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra 400012, IndiaDepartment of Radiation Oncology, Tata Memorial Hospital, Mumbai, Maharashtra 400012, IndiaDepartment of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India; Corresponding authorSummary: Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines.For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721003853Cell-based AssaysImmunologyMicroscopyMolecular BiologyNeuroscience |
| spellingShingle | Ishan Agrawal Nidhi Sharma Shivanjali Saxena S. Arvind Debayani Chakraborty Debarati Bhunia Chakraborty Deepak Jha Surajit Ghatak Sridhar Epari Tejpal Gupta Sushmita Jha Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells STAR Protocols Cell-based Assays Immunology Microscopy Molecular Biology Neuroscience |
| title | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
| title_full | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
| title_fullStr | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
| title_full_unstemmed | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
| title_short | Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
| title_sort | protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells |
| topic | Cell-based Assays Immunology Microscopy Molecular Biology Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721003853 |
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