MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis
MAGI1 is an intracellular adaptor protein that stabilizes cell junctions and regulates epithelial and endothelial integrity. Here, we report that that in endothelial cells MAGI1 colocalizes with paxillin, β3-integrin, talin 1, tensin 3 and α-4-actinin at mature focal adhesions and actin stress fiber...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-01-01
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| Series: | Cell Adhesion & Migration |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19336918.2021.1911472 |
| _version_ | 1818701197433896960 |
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| author | Begoña Alday-Parejo Kedar Ghimire Oriana Coquoz Gioele W. Albisetti Luca Tamò Jelena Zaric Jimmy Stalin Curzio Rüegg |
| author_facet | Begoña Alday-Parejo Kedar Ghimire Oriana Coquoz Gioele W. Albisetti Luca Tamò Jelena Zaric Jimmy Stalin Curzio Rüegg |
| author_sort | Begoña Alday-Parejo |
| collection | DOAJ |
| description | MAGI1 is an intracellular adaptor protein that stabilizes cell junctions and regulates epithelial and endothelial integrity. Here, we report that that in endothelial cells MAGI1 colocalizes with paxillin, β3-integrin, talin 1, tensin 3 and α-4-actinin at mature focal adhesions and actin stress fibers, and regulates their dynamics. Downregulation of MAGI1 reduces focal adhesion formation and maturation, cell spreading, actin stress fiber formation and RhoA/Rac1 activation. MAGI1 silencing increases phosphorylation of paxillin at Y118, an indicator of focal adhesion turnover. MAGI1 promotes integrin-dependent endothelial cells adhesion to ECM, reduces invasion and tubulogenesisin vitro and suppresses angiogenesis in vivo. Our results identify MAGI1 as anovel component of focal adhesions, and regulator of focal adhesion dynamics, cell adhesion, invasion and angiogenesis. |
| first_indexed | 2024-12-17T15:17:01Z |
| format | Article |
| id | doaj.art-5ae7a60c3ea148fd803ffabb79fa0f46 |
| institution | Directory Open Access Journal |
| issn | 1933-6918 1933-6926 |
| language | English |
| last_indexed | 2024-12-17T15:17:01Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj.art-5ae7a60c3ea148fd803ffabb79fa0f462022-12-21T21:43:30ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262021-01-0115112613910.1080/19336918.2021.19114721911472MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesisBegoña Alday-Parejo0Kedar Ghimire1Oriana Coquoz2Gioele W. Albisetti3Luca Tamò4Jelena Zaric5Jimmy Stalin6Curzio Rüegg7University of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgMAGI1 is an intracellular adaptor protein that stabilizes cell junctions and regulates epithelial and endothelial integrity. Here, we report that that in endothelial cells MAGI1 colocalizes with paxillin, β3-integrin, talin 1, tensin 3 and α-4-actinin at mature focal adhesions and actin stress fibers, and regulates their dynamics. Downregulation of MAGI1 reduces focal adhesion formation and maturation, cell spreading, actin stress fiber formation and RhoA/Rac1 activation. MAGI1 silencing increases phosphorylation of paxillin at Y118, an indicator of focal adhesion turnover. MAGI1 promotes integrin-dependent endothelial cells adhesion to ECM, reduces invasion and tubulogenesisin vitro and suppresses angiogenesis in vivo. Our results identify MAGI1 as anovel component of focal adhesions, and regulator of focal adhesion dynamics, cell adhesion, invasion and angiogenesis.http://dx.doi.org/10.1080/19336918.2021.1911472cell adhesionmigrationsignalingextracellular matrixcytoskeletonstress fibers |
| spellingShingle | Begoña Alday-Parejo Kedar Ghimire Oriana Coquoz Gioele W. Albisetti Luca Tamò Jelena Zaric Jimmy Stalin Curzio Rüegg MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis Cell Adhesion & Migration cell adhesion migration signaling extracellular matrix cytoskeleton stress fibers |
| title | MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis |
| title_full | MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis |
| title_fullStr | MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis |
| title_full_unstemmed | MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis |
| title_short | MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis |
| title_sort | magi1 localizes to mature focal adhesion and modulates endothelial cell adhesion migration and angiogenesis |
| topic | cell adhesion migration signaling extracellular matrix cytoskeleton stress fibers |
| url | http://dx.doi.org/10.1080/19336918.2021.1911472 |
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