Determining buffer conditions for downstream processing of VLP-based recombinant hepatitis B surface antigen using multimodal resins in bind-elute and flow-through purification modes

Determining buffer conditions for downstream processing of VLP-based recombinant hepatitis B surface antigen using multimodal resins in bind-elute and flow-through purification modes

Abstract The difficulties in purification of VLP-based recombinant hepatitis B surface antigen (rHBsAg) are mainly emerged from inefficient semi-purification step plus proteins physicochemical properties and these issues make the downstream processing (DSP) very lengthy and expensive. In this study,...

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Bibliographic Details
Main Authors: Maryam Moazami Goodarzi, Reza Jalalirad, Delaram Doroud, Hamidreza Hozouri, Mohammadreza Aghasadeghi, Mahdi Paryan
Format: Article
Language:English
Published: Nature Portfolio 2023-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-37614-y

Internet

https://doi.org/10.1038/s41598-023-37614-y

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