Standardization and evaluation of a duplex real-time quantitative PCR for the detection of Leishmania infantum DNA: a sample quality control approach

Standardization and evaluation of a duplex real-time quantitative PCR for the detection of Leishmania infantum DNA: a sample quality control approach

Abstract INTRODUCTION: Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. The present study describes the optimization and evaluation of a duplex real-time quantitative PCR (qPCR) protocol develo...

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Main Authors: Lays Adrianne Mendonça Trajano-Silva, Rômulo Pessoa-e-Silva, Suênia da Cunha Gonçalves-de-Albuquerque, Rayana Carla Silva de Morais, Cíntia Nascimento da Costa-Oliveira, Tayná Correia de Goes, Milena de Paiva-Cavalcanti
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT)
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Visceral leishmaniasis
Diagnosis
HIV/VL co-infection
Sample quality control
Duplex qPCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017002300350&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017002300350&lng=en&tlng=en

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