Cell-targeted gene modification by delivery of CRISPR-Cas9 ribonucleoprotein complexes in pseudotyped lentivirus-derived nanoparticles

Cell-targeted gene modification by delivery of CRISPR-Cas9 ribonucleoprotein complexes in pseudotyped lentivirus-derived nanoparticles

To fully utilize the potential of CRISPR-Cas9-mediated genome editing, time-restricted and targeted delivery is crucial. By modulating the pseudotype of engineered lentivirus-derived nanoparticles (LVNPs), we demonstrate efficient cell-targeted delivery of Cas9/single guide RNA (sgRNA) ribonucleopro...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Ian Helstrup Nielsen, Anne Bruun Rovsing, Jacob Hørlück Janns, Emil Aagaard Thomsen, Albert Ruzo, Andreas Bøggild, Frederikke Nedergaard, Charlotte Thornild Møller, Thomas Boesen, Søren Egedal Degn, Jagesh V. Shah, Jacob Giehm Mikkelsen
Format: Artikel
Sprache:English
Veröffentlicht: Elsevier 2024-12-01
Schriftenreihe:Molecular Therapy: Nucleic Acids
Schlagworte:
Delivery
LVNP
Gag
virus-like particle, VLP, CRISPR-Cas9
pseudotyping
cell-targeted gene modification
Online Zugang:http://www.sciencedirect.com/science/article/pii/S2162253124002051

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http://www.sciencedirect.com/science/article/pii/S2162253124002051

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