Protocol for drug screening of patient-derived tumor organoids using high-content fluorescent imaging

Summary: High-content imaging of tumor organoids (TOs) treated with therapeutic agents provides detailed cell viability readouts at the organoid level. In contrast, most used protocols provide one number per well. While requiring the use of inverted microscopy with an automated stage, this protocol...

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Bibliographic Details
Main Authors: Brian M. Larsen, Andrea Cancino, Jenna M. Shaxted, Ameen A. Salahudeen
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002878