Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain

Summary: Here, we present fixation-driven chemical crosslinking of exogenous ligands, a protocol to visualize the distribution of exogenously administered small molecules in the mouse brain. We first describe the probe design of the small molecules of interest and the probe microinjection into a liv...

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Bibliographic Details
Main Authors: Takeharu Mino, Hiroshi Nonaka, Seiji Sakamoto, Jae Hoon Oh, Itaru Hamachi
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005221