Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain
Summary: Here, we present fixation-driven chemical crosslinking of exogenous ligands, a protocol to visualize the distribution of exogenously administered small molecules in the mouse brain. We first describe the probe design of the small molecules of interest and the probe microinjection into a liv...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723005221 |
| _version_ | 1797706851844882432 |
|---|---|
| author | Takeharu Mino Hiroshi Nonaka Seiji Sakamoto Jae Hoon Oh Itaru Hamachi |
| author_facet | Takeharu Mino Hiroshi Nonaka Seiji Sakamoto Jae Hoon Oh Itaru Hamachi |
| author_sort | Takeharu Mino |
| collection | DOAJ |
| description | Summary: Here, we present fixation-driven chemical crosslinking of exogenous ligands, a protocol to visualize the distribution of exogenously administered small molecules in the mouse brain. We first describe the probe design of the small molecules of interest and the probe microinjection into a live mouse brain in detail. We then detail procedures for paraformaldehyde-perfusion fixation. This approach is especially useful for imaging-based evaluation of the small-molecule ligands distribution in mouse brain tissue relying on their interaction with endogenous proteins.For complete details on the use and execution of this protocol, please refer to Nonaka et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T05:57:27Z |
| format | Article |
| id | doaj.art-5b2ecef8463447f6bd2e7267fcc774c4 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T05:57:27Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-5b2ecef8463447f6bd2e7267fcc774c42023-09-03T04:24:30ZengElsevierSTAR Protocols2666-16672023-09-0143102555Protocol to visualize the distribution of exogenously administered small molecules in the mouse brainTakeharu Mino0Hiroshi Nonaka1Seiji Sakamoto2Jae Hoon Oh3Itaru Hamachi4Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, JapanDepartment of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan; ERATO (Exploratory Research for Advanced Technology, JST), Tokyo 102-0075, JapanERATO (Exploratory Research for Advanced Technology, JST), Tokyo 102-0075, JapanERATO (Exploratory Research for Advanced Technology, JST), Tokyo 102-0075, JapanDepartment of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan; ERATO (Exploratory Research for Advanced Technology, JST), Tokyo 102-0075, Japan; Corresponding authorSummary: Here, we present fixation-driven chemical crosslinking of exogenous ligands, a protocol to visualize the distribution of exogenously administered small molecules in the mouse brain. We first describe the probe design of the small molecules of interest and the probe microinjection into a live mouse brain in detail. We then detail procedures for paraformaldehyde-perfusion fixation. This approach is especially useful for imaging-based evaluation of the small-molecule ligands distribution in mouse brain tissue relying on their interaction with endogenous proteins.For complete details on the use and execution of this protocol, please refer to Nonaka et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723005221NeuroscienceMolecular/Chemical ProbesChemistry |
| spellingShingle | Takeharu Mino Hiroshi Nonaka Seiji Sakamoto Jae Hoon Oh Itaru Hamachi Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain STAR Protocols Neuroscience Molecular/Chemical Probes Chemistry |
| title | Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| title_full | Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| title_fullStr | Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| title_full_unstemmed | Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| title_short | Protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| title_sort | protocol to visualize the distribution of exogenously administered small molecules in the mouse brain |
| topic | Neuroscience Molecular/Chemical Probes Chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723005221 |
| work_keys_str_mv | AT takeharumino protocoltovisualizethedistributionofexogenouslyadministeredsmallmoleculesinthemousebrain AT hiroshinonaka protocoltovisualizethedistributionofexogenouslyadministeredsmallmoleculesinthemousebrain AT seijisakamoto protocoltovisualizethedistributionofexogenouslyadministeredsmallmoleculesinthemousebrain AT jaehoonoh protocoltovisualizethedistributionofexogenouslyadministeredsmallmoleculesinthemousebrain AT itaruhamachi protocoltovisualizethedistributionofexogenouslyadministeredsmallmoleculesinthemousebrain |