A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger

A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger

Aspergillus niger is a highly versatile fungal strain utilized in industrial production. The expression levels of recombinant genes in A. niger can be enhanced by increasing the copy number. Nevertheless, given the prolonged gene editing cycle of A. niger, a “one-step” strategy facilitating the simu...

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Bibliographic Details
Main Authors: Yangyang Li, Cen Li, Yishan Fu, Quan Zhang, Jianing Ma, Jingwen Zhou, Jianghua Li, Guocheng Du, Song Liu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-06-01
Series:Synthetic and Systems Biotechnology
Subjects:
Multiplex integration
Aspergillus niger
CRISPR-Cas9 homologous direct repair
White colony
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X24000140

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http://www.sciencedirect.com/science/article/pii/S2405805X24000140

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