Development of a Sensitive, Easy and High-Throughput Compliant Protocol for Maize and Soybean DNA Extraction and Quantitation Using a Plant-Specific Universal Taqman Minor Groove Binder Probe

Development of a Sensitive, Easy and High-Throughput Compliant Protocol for Maize and Soybean DNA Extraction and Quantitation Using a Plant-Specific Universal Taqman Minor Groove Binder Probe

We report the optimization of a high-throughput, compliant DNA extraction method that uses standard format 96-well plates and a commercial automated DNA purification system (ABI PRISM<sup>®</sup> 6100 Nucleic Acid PrepStation). The procedure was set up for maize and soybean, the most com...

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Bibliographic Details
Main Authors: Roberto Ambra, Marco Marcelli, Fabio D’Orso
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Genes
Subjects:
plant DNA extraction
silica
chaotropic salts
<i>Zea mays</i>
<i>Glycine max</i>
ribosomal 18S rRNA gene
Online Access:https://www.mdpi.com/2073-4425/14/9/1797

Internet

https://www.mdpi.com/2073-4425/14/9/1797

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