Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing
Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes un...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2023-12-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
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| Online Access: | https://ajs.journals.ekb.eg/article_330118_3415cd4f0035e260b22d7c6bdde5b094.pdf |
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| author | Sally Hassan Mohamed Abdel-Salam ayman hanfy Haytham Abd-Elhalim Nouh Ahmed Emad Metry |
| author_facet | Sally Hassan Mohamed Abdel-Salam ayman hanfy Haytham Abd-Elhalim Nouh Ahmed Emad Metry |
| author_sort | Sally Hassan |
| collection | DOAJ |
| description | Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers |
| first_indexed | 2024-03-07T14:18:22Z |
| format | Article |
| id | doaj.art-5b8e5bf7e9ff48f9806b2e4e4f747a84 |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:56:17Z |
| publishDate | 2023-12-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-5b8e5bf7e9ff48f9806b2e4e4f747a842024-03-07T17:27:33ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852023-12-0131215717110.21608/ajs.2023.198877.1517330118Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome EditingSally Hassan0Mohamed Abdel-Salam1ayman hanfy2Haytham Abd-Elhalim3Nouh Ahmed4Emad Metry5Department of Plant Genetic Transformation, Agricultural Genetic Engineering Research Institute - Agricultural Research Center, Giza, Egypt.Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptGenetics Department, Faculty of Agriculture, Ain Shams University, Cairo, EgyptSchool of Biotechnology, Badr University in Cairo, Badr City, 11829, Cairo, EgyptGenetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.Department of Plant Genetic Transformation, Agricultural Genetic Engineering Research Institute - Agricultural Research Center, Giza, Egypt.Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubershttps://ajs.journals.ekb.eg/article_330118_3415cd4f0035e260b22d7c6bdde5b094.pdfcrisprcold storagecold-induced sweetening (cis)genome editingmonosaccharidesstarch phosphorylase |
| spellingShingle | Sally Hassan Mohamed Abdel-Salam ayman hanfy Haytham Abd-Elhalim Nouh Ahmed Emad Metry Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing Arab Universities Journal of Agricultural Sciences crispr cold storage cold-induced sweetening (cis) genome editing monosaccharides starch phosphorylase |
| title | Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing |
| title_full | Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing |
| title_fullStr | Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing |
| title_full_unstemmed | Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing |
| title_short | Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing |
| title_sort | decreasing of monosaccharide contents resulted from cold induced sweetening in tetraploid potato solanum tuberosum l using genome editing |
| topic | crispr cold storage cold-induced sweetening (cis) genome editing monosaccharides starch phosphorylase |
| url | https://ajs.journals.ekb.eg/article_330118_3415cd4f0035e260b22d7c6bdde5b094.pdf |
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