Quantification of cell energetics in human subcutaneous adipose progenitor cells after target gene knockdown

Summary: Pro-preadipocytes are adipocyte progenitor cells within subcutaneous adipose tissue that are conserved in human adipose tissue with distinct cellular energetics. Here, we detail a protocol to quantify cellular oxygen consumption rates of primary human cells harvested from adipose tissue. We...

Full description

Bibliographic Details
Main Authors: Liang Li, Anya M. Gunewardena, Tenzin Nyima, Brian J. Feldman
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005749