Quantification of cell energetics in human subcutaneous adipose progenitor cells after target gene knockdown
Summary: Pro-preadipocytes are adipocyte progenitor cells within subcutaneous adipose tissue that are conserved in human adipose tissue with distinct cellular energetics. Here, we detail a protocol to quantify cellular oxygen consumption rates of primary human cells harvested from adipose tissue. We...
Main Authors: | Liang Li, Anya M. Gunewardena, Tenzin Nyima, Brian J. Feldman |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723005749 |
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