ELISA- and Activity Assay-Based Quantification of BMP-2 Released <i>In Vitro</i> Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan

ELISA- and Activity Assay-Based Quantification of BMP-2 Released <i>In Vitro</i> Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan

Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable <i>in vitro</i> release results, the robustnes...

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Bibliographic Details
Main Authors: Julius Sundermann, Steffen Sydow, Laura Burmeister, Andrea Hoffmann, Henning Menzel, Heike Bunjes
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Pharmaceutics
Subjects:
bone morphogenetic protein 2
BMP-2
chitosan
release quantification
matrix effects
Online Access:https://www.mdpi.com/1999-4923/13/4/582

Internet

https://www.mdpi.com/1999-4923/13/4/582

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