The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress
Living in the earth’s oxygenated environment forced organisms to develop strategies to cope with the damaging effects of molecular oxygen known as reactive oxygen species (ROS). Here we show that Per2, a molecular component of the mammalian circadian clock, is involved in regulating a cell’s respons...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-01-01
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| Series: | Frontiers in Neurology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fneur.2014.00289/full |
| _version_ | 1818367825754980352 |
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| author | Maria Chiara Magnone Sonja eLangmesser April Candice Bezdek Tiziano eTallone Sandro eRusconi Urs eAlbrecht |
| author_facet | Maria Chiara Magnone Sonja eLangmesser April Candice Bezdek Tiziano eTallone Sandro eRusconi Urs eAlbrecht |
| author_sort | Maria Chiara Magnone |
| collection | DOAJ |
| description | Living in the earth’s oxygenated environment forced organisms to develop strategies to cope with the damaging effects of molecular oxygen known as reactive oxygen species (ROS). Here we show that Per2, a molecular component of the mammalian circadian clock, is involved in regulating a cell’s response to oxidative stress. Mouse embryonic fibroblasts (MEFs) containing a mutation in the Per2 gene are more resistant to cytotoxic effects mediated by ROS than wild type cells which is paralleled by an altered regulation of bcl-2 expression in Per2 mutant MEFs. The elevated survival rate and alteration of NADH/NAD+ ratio in the mutant cells is reversed by introduction of the wild type Per2 gene. Interestingly, clock synchronized cells display a time dependent sensitivity to paraquat, a ROS inducing agent. Our observations indicate that the circadian clock is involved in regulating the fate of a cell to survive or to die in response to oxidative stress, which could have implications for cancer development and the aging process. |
| first_indexed | 2024-12-13T22:58:13Z |
| format | Article |
| id | doaj.art-5be31b4352794d61a1534b5f7e86acb4 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-13T22:58:13Z |
| publishDate | 2015-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-5be31b4352794d61a1534b5f7e86acb42022-12-21T23:28:28ZengFrontiers Media S.A.Frontiers in Neurology1664-22952015-01-01510.3389/fneur.2014.00289127293The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stressMaria Chiara Magnone0Sonja eLangmesser1April Candice Bezdek2Tiziano eTallone3Sandro eRusconi4Urs eAlbrecht5University of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgUniversity of FribourgLiving in the earth’s oxygenated environment forced organisms to develop strategies to cope with the damaging effects of molecular oxygen known as reactive oxygen species (ROS). Here we show that Per2, a molecular component of the mammalian circadian clock, is involved in regulating a cell’s response to oxidative stress. Mouse embryonic fibroblasts (MEFs) containing a mutation in the Per2 gene are more resistant to cytotoxic effects mediated by ROS than wild type cells which is paralleled by an altered regulation of bcl-2 expression in Per2 mutant MEFs. The elevated survival rate and alteration of NADH/NAD+ ratio in the mutant cells is reversed by introduction of the wild type Per2 gene. Interestingly, clock synchronized cells display a time dependent sensitivity to paraquat, a ROS inducing agent. Our observations indicate that the circadian clock is involved in regulating the fate of a cell to survive or to die in response to oxidative stress, which could have implications for cancer development and the aging process.http://journal.frontiersin.org/Journal/10.3389/fneur.2014.00289/fullApoptosisParaquatCircadian clockBcl-2adenovirusp53 |
| spellingShingle | Maria Chiara Magnone Sonja eLangmesser April Candice Bezdek Tiziano eTallone Sandro eRusconi Urs eAlbrecht The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress Frontiers in Neurology Apoptosis Paraquat Circadian clock Bcl-2 adenovirus p53 |
| title | The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress |
| title_full | The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress |
| title_fullStr | The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress |
| title_full_unstemmed | The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress |
| title_short | The mammalian circadian clock gene Per2 modulates cell death in response to oxidative stress |
| title_sort | mammalian circadian clock gene per2 modulates cell death in response to oxidative stress |
| topic | Apoptosis Paraquat Circadian clock Bcl-2 adenovirus p53 |
| url | http://journal.frontiersin.org/Journal/10.3389/fneur.2014.00289/full |
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