Phenotype-Independent Isolation of Interspecies Saccharomyces Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting

Phenotype-Independent Isolation of Interspecies Saccharomyces Hybrids by Dual-Dye Fluorescent Staining and Fluorescence-Activated Cell Sorting

Interspecies hybrids of Saccharomyces species are found in a variety of industrial environments and often outperform their parental strains in industrial fermentation processes. Interspecies hybridization is therefore increasingly considered as an approach for improvement and diversification of yeas...

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Bibliographic Details
Main Authors: Arthur R. Gorter de Vries, Charlotte C. Koster, Susan M. Weening, Marijke A. H. Luttik, Niels G. A. Kuijpers, Jan-Maarten A. Geertman, Jack T. Pronk, Jean-Marc G. Daran
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Microbiology
Subjects:
FACS
Saccharomyces eubayanus × Saccharomyces cerevisiae hybrids
heterosis
marker-free mating
lager beer brewing
non-GMO
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00871/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00871/full

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