Multiple gene substitution by Target-AID base-editing technology in tomato
Abstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-77379-2 |
| _version_ | 1818749223304167424 |
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| author | Johan Hunziker Keiji Nishida Akihiko Kondo Sanae Kishimoto Tohru Ariizumi Hiroshi Ezura |
| author_facet | Johan Hunziker Keiji Nishida Akihiko Kondo Sanae Kishimoto Tohru Ariizumi Hiroshi Ezura |
| author_sort | Johan Hunziker |
| collection | DOAJ |
| description | Abstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions. |
| first_indexed | 2024-12-18T04:00:22Z |
| format | Article |
| id | doaj.art-5c452f7144bc471a93a6b09ad8de7141 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:00:22Z |
| publishDate | 2020-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5c452f7144bc471a93a6b09ad8de71412022-12-21T21:21:41ZengNature PortfolioScientific Reports2045-23222020-11-0110111210.1038/s41598-020-77379-2Multiple gene substitution by Target-AID base-editing technology in tomatoJohan Hunziker0Keiji Nishida1Akihiko Kondo2Sanae Kishimoto3Tohru Ariizumi4Hiroshi Ezura5Graduate School of Life and Environmental Sciences, University of TsukubaGraduate School of Science, Technology and Innovation, Kobe UniversityGraduate School of Science, Technology and Innovation, Kobe UniversityInstitute of Vegetable and Floricultural Science, NAROFaculty of Life and Environmental Sciences, University of TsukubaFaculty of Life and Environmental Sciences, University of TsukubaAbstract The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions.https://doi.org/10.1038/s41598-020-77379-2 |
| spellingShingle | Johan Hunziker Keiji Nishida Akihiko Kondo Sanae Kishimoto Tohru Ariizumi Hiroshi Ezura Multiple gene substitution by Target-AID base-editing technology in tomato Scientific Reports |
| title | Multiple gene substitution by Target-AID base-editing technology in tomato |
| title_full | Multiple gene substitution by Target-AID base-editing technology in tomato |
| title_fullStr | Multiple gene substitution by Target-AID base-editing technology in tomato |
| title_full_unstemmed | Multiple gene substitution by Target-AID base-editing technology in tomato |
| title_short | Multiple gene substitution by Target-AID base-editing technology in tomato |
| title_sort | multiple gene substitution by target aid base editing technology in tomato |
| url | https://doi.org/10.1038/s41598-020-77379-2 |
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